5 - Fluorouracil - Silk Fibroin - Chitosan Microcapsules Prepared

5-Fluorouracil (5-FU) is an anticancer agent that is widely used in the clinical treatment of several solid cancers, such as breast, colorectal, liver and brain cancer. Because of its high rate of metabolism in the body, the maintenance of a high serum concentration improves its therapeutic activity, but this requires its continuous administration. However, concentration above a certain limit produces a series of toxic effects. Via changing the medicine preparation form to prolong medicine's effective time and play down medicine dosage have realistic significance. This paper discussed the preparation and the property of 5-FU loaded microcapsules with cross linked chitosan and silk fibroin, as well as drug in microcapsule releasing capability. The results give certain theory-base for the microcapsule preparation of 5-FU.In the emulsion system, liquid proffin as continuous phase, Span as emulsifier, glutaradehyde as crosslinking agent, the silk fibroin-chitosan microcapsule with 5-FU wrapped in is prepared. Microcapsules are prepared using complex coacervation method. In order to get optimized preparation parameters, different factors are selected to evaluation the influence of different formulation in the preparation of microcapsule. By the single-factor and the orthogonal test, the concentration of silk fibroin, emulsifier concentration, glutaraldehyde amount and ratio of oil phase and water phase have done the most important effect to the formulation of microcapsules. Optimize experiment receive the furthest fit technical parameters: the concentration of silk fibroin is 3%, emulsifier concentration is 2ml, glutaraldehyde amount is 2ml, ratio of H2O / Oil (V:V) is 1:5. Under the optimum conditions, microcapsules are in good shape and approximatively global, the mean diameter is about 48.98μm.Through testing the microcapsule's property, the results indicate that: silk fibroin and chitosan are cross linked by strong coactions, 5-FU is firmly embedded in capsule wall material; the concentration of 5-FU, glutaradehyde and emulsifier has done some effect on encapsulation efficiency and drug contents, 5-FU concentration is the most important influencing factor of the total; the mean recovery rate of microcapsules is 97.25%; the experiment of releasing drug explains that 5-FU-silk fibroin and chitosan microcapsules possess more obvious sustained releasing property than 5-FU and 5-FU-chitosan microcapsules, which are accord with the test request. Silk fibroin and chitosan are profitable capsule wall material to embed 5-FU.Through optimizing preparation conditions, microcapsules are in good shape and approximatively global, particle size is uniform. Microcapsules may prove better sustained release of drugs.