Discovery Of Effective Components Of Tibetan Medicine For Lowering Blood Pressure And Lipids And Research On Its Formulation

Tibetan medicine has been used for thousands of years.Through thousands years of clinical application and modern pharmacological research in recent decades,the effect of Tibetan medicine is approved.According to the latest survey,there are 2172 herbal medicines,214 animal drugs and over 50 mineral drugs used in Tibetan medicine,but only 304 herbs are used in clinic commonly.In this study,fragment-based searching and pharmacophore-based searching were used basing on Common Tibetan medicine Database to screen active ingredients for anti-hypertension and anti-hyperlipidemia.After this,association rule was applied to discover Tibetan medicine pairs for treating hypertension and hyperlipidemia.Based on the results of two parts,new prescriptions were discussed in this thesis.1.Common Tibetan medicine Construction.MDL ISIS/Base was applied to build a database of Common Tibetan medicine.The database includes 148 Tibetan drugs,and was comprised of drug description data,compound information and literature information.Drug description data mainly contains 11 attributes,such as property,origin,pharmacological effects,function and symptom and clinical application of Tibetan medicine.Compound information includes name in both Chinese and English,molecular formula,physicochemical properties and so on.Literature information is the record of modern research of Tibetan medicine.2.Active ingredients screening and prescriptions design for anti-hypertension and anti-hyperlipidemia.Based on Common Tibetan medicine Database,database searching,fragment-based screening,similarity searching and pharmacophore-based screening were used to screen potential active ingredients with the activity of anti-hypertension and anti-hyperlipidemia.Herbs which contains these active ingredients was retraced.53 potential anti-hypotensive ingredients and 13 potential anti-hyperlipidemia ingredients were obtained by database searching,which extracted from 36 and 22 Tibetan medicines,respectively.By using fragment-,pharmacophore-and targets-based searching techniques there were 107 anti-hypertensive ingredients and 265 anti-hyperlipidemic ingredients obtained,which delt with 62 and 56 Tibetan medicines,respectively.In order to avoid false positive results from simulations,association rule was also applied to analyze the clinical applying rules of Tibetan medicine for anti-hypertension and anti-hyperlipidemia,and 6 anti-hypotensive Tibetan medicine pairs and 8 anti-hyperlipidemia Tibetan medicine pairs were obtained.Combined the results of the active ingredients screening and the association rule analysis,2 anti-hypotensive prescriptions and 2 anti-hyperlipidemia prescriptions were designed in the guidance of Tibetan Medicine theory.