The Isolation And Purification Of Chicory Polysaccharide And Its Lipid-lowering Effect On Rats

Cichorium intybus L.belongs to Cichorium L.family,and it is a perennial medicinal and edible herbs,chicory roots fleshy,short and thick.Chicory is mainly distributed in Europe,Asia,North Africa,China's Beijing,Liaoning,Xinjiang,Jiangxi and other provinces and cities are also distributed,and it is the Uighur and Mongolian commonly used herbs.Chicory is rich in polysaccharides,alkaloids,terpenoids and other biological active substances,in which the study of chicory polysaccharide has attracted the attention of many scholars.In this paper,the extraction technology of chicory polysaccharide,separation and purification technology,monosaccharide composition,antioxidant activity in vitro and its hypolipidemic effect,in order to lay the foundation for the industrialization of chicory polysaccharides.The main conclusions were as follows:1.The microwave-assisted enzyme extraction of chicory polysaccharides was carried out by Response Surface Method(RSM)using Design Expert 8.05 Professional Software.The optimal extraction conditions were obtained as follows:solid-to-liquid ratio of 1:30(g/mL),cellulose dosage 2.5%,enzymolysis time 105min,enzymolysis temperature 55 ?,pH5.5,microwave time 3min and microwave power 480W,rapid heating up to 100?,after enzymolysis,destroy enzyme 15 min,adjust the pH to neutral,and then hot water extraction,the extraction yield of polysaccharides from chicory was(44.369+0.25)%.2.Savage method,TCA method,hydrochloric acid method and enzyme method were used to study the method of removing protein from chicory polysaccharide.The results showed that the enzymatic method was the best method to remove protein.DEAE-52 cellulose ion exchange column chromatography was used to separate the chicory polysaccharides,and two polysaccharides were obtained and named as CIP-1 and CIP-2.The two components of CEP-1 and CIP-2 were further purified by SephadexG-200 gel-column chromatography.CIP-1 and CIP-2 were eluted by SephadexG-200 column chromatography to obtain a single peak,CIP-1 and CIP-2 accounted for 84.12%and 15.88%of the total sugar content,respectively.The purity,molecular weight,monosaccharide composition and structure of CIP-1 were analyzed by UV scanning(UV-vis),high performance gel chromatography(HPGPC),gas chromatography-mass spectrometry(GS-MS)and infrared spectroscopy(IR),the results showed that CEP-1 had no absorption peak of nucleic acid and protein in the wavelength of 270-280 nm;the relative molecular weight of CIP-1 was 8511D;CIP-1 was a miscellaneous polysaccharides and it were composed of sorbitol,glucose,fructose and glucitol,miscellaneous polysaccharides;the infrared spectra showed that the CIP-1 have the characteristic absorption peaks of polysaccharides.3.By determinate the effect of CIP-1 on DPPH radical,hydroxyl radical and reducing power,the result showed that CIP-1 had strong scavenging activity on DPPH free radical,hydroxyl radical and the scavenging ability of DPPH was stronger than that of ·OH,and the scavenging effect increased with the increase of CIP-1 concentration.4.SD male rats were used to study the function of CIP-1 in reducing blood lipids.The rats were randomly divided into 6 groups:blank control group(NC),high fat model group(HM,positive control group(PC),high dose(HD),middle dose group(MD),low dose group(LD).The different concentrations of CIP-1 were chronically administered to rats for 35d,and then measured the changes of body weight,food intake,liver index,total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),malonaldehyde(MDA),superoxide dismutase(SOD),alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum of rats and liver homogenate malondialdehyde(MDA),superoxide dismutase(SOD),total antioxidant capacity(T-AOC).The results showed that CIP-1 had a significant effect on reducing blood lipid and protect the liver in hyperkinemia rats.