An Experimental Study Of Yifeiyin Combined With CIK Cells To Interfere With Immune Escape Of Lung Cancer A549 Cells

Objective: Through the analysis of proliferation and apoptosis of lung cancer cells,the expression of transforming growth factor-? and VEGF,to investigate the influence of the immune escape of lung cancer cells at the molecular level by Yifei decoction and CIK cells alone or in combination.To provide a total of experimental data support for clinical treatment,and develop new treatment joint models.Methods:1.The effect of different concentrations of Yifei decoction-containing serum on the proliferation of A549 cells was observed by CCK-8 method.2.Flow cytometry was used to detect the effect of different concentrations of drug-containing serum on apoptosis of A549 cells.3.The effects of drug-containing serum combined with CIK cells on proliferation and apoptosis of A549 cells were detected by CCK-8 method and flow cytometry.4.The effect of drug-containing serum combined with CIK cells on the expression of transforming growth factor-? and VEGF in A549 cells was detected by ELISA.Results:(1)Compared with the control group(10% FBS group and drug-free serum group),the serum concentration of Yifei Decoction had obvious proliferation inhibition effect on A549 cells(P<0.05).The inhibition rate increased with increasing drug concentration,compared with other groups(P<0.05).(2)The effects of serum containing Yifei Decoction on apoptosis of lung cancer A549 cells were detected by flow cytometry.Different concentration of drug-containing serum was compared with the blank control group(P<0.05),the results were statistically different.There were significant differences in apoptotic rate among the concentrations of 5%,10%,and 15% drug-containing serum groups,P<0.05.The apoptotic rate had no difference between 15% and 20%drug-containing serum(P>0.05).(3)Yifei decoction and CIK alone or in combination compared with the control group showed significant inhibition of proliferation and induction of apoptosis.There was no significant difference in the inhibition rate and apoptotic rate of A549 between theYifei decoction+CIK group and the CIK group(P>0.05),but they were better than the Yifei decoction group(P<0.05).(4)The Yifeiyin group and the CIK group had inhibitory effects on the secretion of TGF-? and VEGF compared with the control group when used alone or in combination(P<0.05).Yifei decoction+CIK group showed the most obvious inhibitory effect on TGF-?,compared with other groups(P<0.05).The inhibitory effect of Yifei Decoction combined with CIK group on VEGF was not significantly different from that of Yifei Decoction and CIK alone(P>0.05).Conclusion: 1.Yifei Decoction-containing serum has the effect of inhibiting proliferation and inducing apoptosis of lung cancer A549 cells.2.The combination of Yifei Decoction-containing serum and CIK cells could not enhance the proliferation inhibition and induce apoptosis of A549 cells.3.Yifei Decoction-containing serum can inhibit the secretion of TGF-? and VEGF in A549 cells.4.The serum containing Yifei Decoction can enhance the inhibitory effect on TGF-? when combined with CIK cells,but it has no obvious enhancement effect on the inhibitory effect of VEGF.