| Background:Cancer is one of the leading causes of death in the world,and cancer poses an increasing challenge to the world due to its complex and diverse risk factors.Gastric cancer is the most common type of upper gastrointestinal cancer.Due to its high incidence,low early diagnosis rate and poor prognosis,gastric cancer has become a serious threat to human health.Invasion and metastasis of gastric cancer are two of the main reasons for its poor prognosis.Gastric cancer cells through the regulation of various molecular mechanisms and multi-step physiological processes lead to invasion and metastasis of tumor cells to the surrounding and distant tissues.With the deeper understanding and research of tumors,many molecules and signaling pathways have been found to regulate the invasion and metastasis of gastric cancer cells.Galectin-1(Gal-1)is a lectin protein commonly found in mammalian cells.It has been found in many types of tumor cells and can participate in the biology of various tumors.The process is closely related to the occurrence and development of tumors.Studies have shown that Galectin-1 can be highly expressed in gastric cancer,and it can regulate a variety of biological behaviors such as angiogenesis in gastric cancer,and it suggests poor prognosis.However,the exact biological function of Galectin-1 in gastric cancer cells and its mechanism have not been fully elucidated.C-X-C chemokine receptor type 4(CXCR4)is a small molecule that binds to chemokines and it can directly abduct cells.It can respond to inflammation,wound repair,and tumor progression,and it is highly expressed in a variety of tumor cells which can suggest a poor prognosis.However,whether the expression of CXCR4 in gastric cancer cells is related to Galectin-1 and whether it has a regulatory effect on the prognosis of gastric cancer has not been clearly reported.Purpose and meaning:This study mainly explores the expression and correlation of Galectin-1 and CXCR4 in gastric cancer tissues and cells through basic experimental studies.Study the regulation of Galectin-1 on the expression of CXCR4 protein and the regulation of invasion and metastasis of gastric cancer cells.Discuss the mechanism of Galectin-1 in the regulation of invasion and metastasis of gastric cancer.Methods:1.Immunohistochemistry and real-time quantitative PCR were used to detect the expression of Galectin-1 and CXCR4 in tissue samples from 103 patients with gastric cancer.2.Transfected BGC-823 gastric cancer cells with lentivirus to construct a cell model of Galectin-1 knockdown and overexpression.Cell invasion assay and scratch assay were used to detect the invasion and metastasis of gastric cancer cells with different expression levels of Galectin-1 and CXCR4.3.The nude mice were injected subcutaneously with gastric cancer cells with different expression levels of Galectin-1 to construct a mouse subcutaneous tumor model to detect the effect of Galectin-1 on the proliferation of gastric cancer cells.Results:High expression of Galectin-1 and CXCR4 were associated with poor prognosis of patients with gastric cancer.Overexpression of Galectin-1 up-regulated CXCR4 expression and enhanced the invasion and metastasis of BGC-823 cells.After knocking down Galectin-1,the expression of CXCR4 was decreased,and the invasion and metastasis ability of gastric cancer cells were also inhibited.Similar results were obtained after the gastric cancer cells were treated with AMD3100—the inhibitor of CXCR4.Overexpression of Galectin-1 promoted the growth of subcutaneous tumors in gastric cancer cells.Conclusions:Galectin-1 promotes the invasion and metastasis of gastric cancer cells by up-regulating the mechanism of CXCR4.Our study suggests that Galectin-1 and CXCR4 may be potential targets for the treatment of gastric cancer. |
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