Study On The Changes Of Peripheral Blood Mononuclear Cell Subsets And Key Cytokines In Patients With Coronary Heart Disease And Heart Failure With Qi Deficiency And Blood Stasis Syndrome

Objective:In this study,peripheral blood monocyte subsets and plasma MCP-1,IL-1?,and IL-6 levels were measured by flow cytometry in patients with coronary heart disease and heart failure.Heart failure patients with coronary heart disease were compared with normal controls about the differences in the level of the above indicators.At the same time,research the correlation between coronary heart disease patients with qi deficiency and blood stasis syndrome and non-qi deficiency and blood stasis syndrome patients with the above indicators.Methods:From October 2017 to February 2018,the Department of Cardiology of Guang'anmen Hospital of China Academy of Chinese Medical Sciences was finally included in 101 patients with coronary heart disease and was included in the health volunteers of the Southern District Medical Center of Guang'anmen Hospital of China Academy of Chinese Medical Sciences.Referring to the "Consensus of Chronic Heart Failure Chinese Medicine Experts in Diagnosis and Treatment" in 2014 and the patients with heart failure of coronary heart disease were divided into the Qi Deficiency Blood Stasis Syndrome group(65cases)and the non-QI Deficiency Blood Stasis Syndrome group(36 cases).All patients were taken intravenous blood in the early morning with EDTA anticoagulant tube.The proportion of monocyte subsets was determined by flow cytometry;plasma MCP-1,IL-1?,and IL-6 levels were determined by enzyme-linked immunosorbent assay.At the same time,clinical cardiac ultrasound data and relevant clinical test data were collected.The data obtained were statistically analyzed using SPSS20.0.Results:1.There was no difference between CD14++CD16+and CD14+CD16++in patients with coronary heart disease and heart failure.The level of CD14+CD16+ increased and the difference was statistically significant(P<0.01).2.The plasma levels of MCP-1,IL-1? and IL-6 in patients with coronary heart disease and heart failure were significantly higher than those in healthy controls(P<0.01).3.Compared with non-qi deficiency and blood stasis syndrome group,there was no difference in the levels of CD14+CD16-and CD14++CD16+ in patients with coronary heart disease and heart failure caused by qi deficiency and blood stasis syndrome.The difference was statistically significant(P<0.05).4.The levels of plasma MCP-1,IL-1? and IL-6 in patients with heart failure and qi deficiency and blood stasis syndrome were significantly higher than those in non-qi deficiency and blood stasis syndrome(P<0.05).5.Coronary heart disease heart failure Qi deficiency and blood stasis syndrome and CD14++CD16-,CD14++ CD16+,MCP-1,IL-6 were positively correlatedConclusions:1.The level of CD14+ CD16+ subpopulation in patients with coronary heart disease is increased.2.The levels of MCP-1,IL-1? and IL-6 in patients with coronary heart failure are increased.3.The levels of CD14+ CD16-,CD14++ CD16+ subpopulations increased in patients with heart failure and Qi deficiency and blood stasis syndrome4.The levels of MCP-1,IL-1? and IL-6 in plasma of patients with heart failure and qi deficiency and blood stasis syndrome are increased5.Coronary heart disease heart failure Qi deficiency and blood stasis syndrome are positively correlated with CD14++ CD16-,CD14++ CD16+,MCP-1,IL-6.