Study On The Expression Of Myeloid-derived Immunosuppressive Cells (MDSCs) And Regulatory T Cells (Tregs) In The Peripheral Blood Of Prostate Cancer Patients

Objective: To investigate the relationship between Myeloid-derived suppressor cells(MDSCs)and regulatory T cells(Tregs)and prostate cancer.To analyze The expression and proportion of MDSCs and Tregs in peripheral blood mononuclear cells(PBMCs)of prostate cancer patients and healthy volunteers.To investigate the role of MDSCs and Tregs in the tumorigenesis and immunotherapy of prostate cancer through statistical analysisMethods: A total of 35 cases of hospitalized prostate cancer patients of Department of Urology,Affiliated Hangzhou First People's Hospital,Nanjing Medical University were included in the study.All the patients were divided into high-risk group,medium-risk group and low-risk group according to pathology and PSA.Eight healthy volunteers were set as the control group.After collecting 6ml peripheral blood of patients and volunteers,peripheral blood mononuclear cells were selected.Then flow cytometry was performed after corresponding immunofluorescence staining of G-MDSCs?M-MDSCs and Tregs of PBMCs.Through this we obtained the expression and proportion of G-MDSCs,M-MDSCs and Tregs and after statistical analysis we studied if there was statistical significant difference in expression ratio between experimental and control groups,and whether the difference in expression ratio between each experimental group was statistically significant.Results: Members of experimental group and control group were all male whose general clinical data such as age showed no statistical difference.Compared to control group,patients of experimental group had a significant increase of G-MDSCs,M-MDCS and Tregs of PBMCs.There was significant difference in the increase of G-MDSCs in high-risk group(P<0.01)and medium-risk group(P<0.01)compared to control group,and low-risk group(P>0.05)did not have a significant increase.The proportion of G-MDSCs was definitely correlated with the degree of tumor risk while the difference was not statistically significant between each group(P>0.05).The increase of M-MDSCs was statistically significant in medium-risk group(P<0.01),while high-risk(P>0.05)and low-risk(P>0.05)group both had no significant increase,and there was no statistical difference between each experimental group.Comparing to the control group,there was an extremely increase of Tregs in high-risk group(P<0.001),and medium-risk group had a significant increase(P<0.01),while low-risk group had no significant increase.High-risk group showed a significant increase of Tregs compared to both medium-risk group(P<0.01)and low-risk group(P<0.01),and there was no statistical difference between medium-risk group and low-risk group(P>0.05).Conclusions: MDSCs and Tregs inhibit immune response,construct tumor microenvironment and participate in tumor immune escape during the development of prostate cancer.There was a statistical increase of MDSCs and Tregs in high-risk patients of prostate cancer,and therefore they can be expected to become a new target for the targeted treatment of prostate cancer,but it requires more research.