The Expression And Significant Of Myeloid Derived Suppressor Cells And Regulatory T Cells In Peripheral Blood And Carcinoma Tissues Of Bladder Urothelium Cancer Patients

Objective:The current paper aims to investigate expression level of myeloid derived suppressor cells(MDSC) and regulatory T cells(Treg) in peripheral anticoagulated blood, bladder carcinoma tissues and pericarcinomatous tissue of bladder urothelium cancer patients, discusses its relationship with clinical pathological characteristics of bladder cancer and the correlations between them. The roles of these cells in bladder urothelium carcinoma were also investigated.Methods:We selected39patients with bladder urothelium cancer in the Department of Urology, the Second Affiliated Hospital of Kunming Medical University from December2012to November2013and20cases of healthy volunteers from Center of Health Examination, their peripheral anticoagulated blood were collected; The carcinoma tissues and2cm bladder tissue speciments outside the carcinoma which radical resection was carried out in39bladder urothelium cancer patients were collected. Flow cytometry was employed to determine the expression level of CD14-CD11b+CD33+MDSC and CD4+CD25+Foxp3+Treg.Results:1. In peripheral blood of bladder urothelium cancer patients, the expression level of MDSC and Treg was significantly higher than that of healthy volunteers, the difference was statistically significant(p<0.05);2. In the bladder carcinoma tissues and2cm bladder tissue speciments adjacent to carcinoma, the expression level of MDSC and Treg of the carcinoma tissues was significantly higher than that of normal tissues adjacent to the tumors, the difference was statistically significant(p<0.05);3. The MDSC and Treg expression level in the peripheral blood, the bladder carcinoma tissues and2cm bladder tissue speciments outside the carcinoma was correlated with the clinical stage, histological grades and presence of lymph node metastasis in the bladder cancer patients(p<0.05), MDSC and Treg expression level was not correlated with ages, gender, tumor diameters, tumor number or tumor recurrence (P>0.05); Compared to the clinical stage, presence of lymph node metastasis and histological grade, correlation of clinical stage and MDSC, Treg expression level is greater(p<0.05).4. The expression level between MDSC and Treg in the peripheral blood, bladder cancer tissues and para-carcinoma tissue specimens of patients was positively correlated, between each pair of date were statistically significant(P<0.05).5The expression level of MDSC in the peripheral blood and tumor tissue of patients with bladder urothelium cancer was higher in para-carcinoma tissue(p<0.05); The expression level of Treg in the peripheral blood and para-carcinoma tissue of patients with bladder urothelium cancer was lower in tumor tissue (p<0.05);Conclusion:The increase of MDSC and Treg in peripheral blood and tumor foci of bladder urothelium cancer patients may cause immune escape important role in the development of bladder urothelium cancer. The role of MDSC and Treg in the tumor biology of bladder cancer might have potential therapeutic implications.