Study On The Effect Of Dioscin On TNF-? And IL-1? Of Sciatic Nerve In Rats With Painful Diabetic Peripheral Neuropathy

Purpose: The aim of this study was to investigate the therapeutic effects and effects of Dioscorea zingiberensis extract on the inflammatory factors TNF-? and IL-1? in the sciatic nerve of rats with painful diabetic peripheral neuropathy.In-depth study of the target of single-flavor Chinese medicine,combined with painful diabetic peripheral neuropathy,focus on the pathological process of peripheral nerve inflammatory cell infiltration,in this role to study its possible molecular mechanism of action,providing new means and ideas for clinical treatment.Material and method: Male 136 SPF Wistar rats,9-10 weeks old,weighing 180-200 g,and fed for one week.Twenty-four rats were randomly selected and divided into 3 groups,which were 2w,4w,8w,and 8 in each group.The remaining 112 were fasted for 12 hours,and a single intraperitoneal injection of 2% streptozotocin solution(53 mg/kg,0.1 mol/l of p H 4.2)prepared from citrate buffer was used to induce diabetes and diosgenin was administered.Or prednisone for 8 weeks.Or prednisone for 8 weeks.Randomly sorted into 2w,4w,8w PDPN group,low dose group,high dose group,prednisone group.Observe the daily mental state,blood sugar,body weight,pain threshold and nerve conduction velocity of the rats after administration;The sciatic nerve was isolated to observe the changes of nerve fibers,axons and myelin.The effects of diosgenin on the expression of TNF-? and IL-1? in sciatic nerve of rats with painful diabetic peripheral neuropathy were detected by immunohistochemistry or immunofluorescence.Results:1.Compared with the normal control group,the PDPN group had heavy odor,dull and dull coat,slow movement speed,poor reaction ability,increased food intake and urine output,poor mental state,and lying on the back.The pupil is grayish and other symptoms.The above conditions occurred in the remaining treatment groups,but the symptoms were alleviated compared with the PDPN group.Among them,the daily state improvement of the high-dose group and the prednisone group was more obvious;2.Compared with the normal control group,the blood glucose level of each model group was significantly increased(P<0.01).There was no significant difference in blood glucose between the treatment group and the PDPN group(P>0.05).3.Compared with the normal control group,the body weight of each model group decreased significantly(P<0.01).There was no significant difference in body weight between the treatmentgroup and the PDPN group(P>0.05).4.Compared with the normal control group,the MNCV of the sciatic nerve in the PDPN group was decreased(P<0.01);the MNCV in the treatment group was significantly higher than that in the PDPN group(P<0.01),and the motor nerve conduction velocity was increased.5.The latency of PDPN group was higher than that of normal group(P<0.01).At 2 weeks,the response latency of high dose group and prednisone group decreased compared with PDPN group(P<0.05);at 4 weeks and 8 weeks Compared with the PDPN group,the response latency of the rats in each treatment group was significantly decreased(P<0.01),and the latency of the rats in the low-dose group and the prednisone group was higher than that in the high-dose group(P<0.05).The latency of the rats in the prednisone group was lower than that in the low-dose group(P<0.05).6.In the PDPN group,the myelin sheath of the myelinated nerve fibers is thin,demyelinated,loose and unorganized,and the lamellar space is enlarged and separated from each other,and obvious signs of axonal atrophy are seen.The damaged nerve fibers in the yam saponin treatment group were partially recovered.7.8 weeks,the levels of TNF-? and IL-1? in the sciatic nerve of PDPN group were significantly higher than normal(P<0.01).The levels of TNF-? and IL-1? in rat sciatic nerve were significantly lower in each treatment group than in PDPN(P<0.01);the high-dose group had lower TNF-? and IL-1? levels than the low-dose sciatic nerve(P<0.01),suggesting amelioration of pathological changes in diabetic rats.Conclusion:1.Dioscorea zingiberensis extract diosgenin can increase the speed of motor nerve transmission in PDPN rats and improve nerve damage in PDPN rats.2.Dioscorea zingiberensis extract diosgenin can reduce the latency of the pain threshold of PDPN rats,has the effect of relieving pain,and protects PDPN rats.3.Dioscorea zingiberensis extract Dioscin can improve the pathological morphology of the sciatic nerve in the PDPN rat group and protect and repair the damaged sciatic nerve.4.The high-dose and low-dose groups of Dioscorea zingiberensis extract could inhibit the expression of TNF-? and IL-1? in the sciatic nerve of PDPN rats,and the high dose effect was more obvious,suggesting that Dioscorea zingiberensis extract can effectively improve The level of sciatic nerve inflammatory factor in PDPN rats,which in turn reduces peripheral nerve damage.