| Objective:In the course of this experiment,rats were used as experimental subjects to use nerve pliers to cause damage to the sciatic nerves of rats and establish models.Secondly,the two points of the "Huantiao" point and the "Wei Zhong" point of the rats were acupuncture by using a 2Hz electric needle.Therefore,The effects of different acupoints on the repair function of sciatic nerve injury were further analyzed.It is mainly analyzed from experimental data on Neurophysiology reports,staining of nerve HE,nerve transmission electron microscopy,immunohistochemistry and immunofluorescence detection of "NCAM" in the ganglion of L4-L5 ridge in rats,"CNTF" and Xuwang.The function index of cells and sciatic nerves confirmed the effectiveness of acupuncture in the treatment of sciatic nerve damage,and the use of "NCAM","CNTF" and Schwann cells as indicators to evaluate the mechanism of acupuncture on damage nerve repair.Material and method:Refer to the previous clamp method to make this experimental model.Using 80clean-grade healthy rats,weighing 250 ± 20 g,and both male and female in age from February to March,they were randomly divided into four groups: false surgery group(normal group),model group,ring jump group and committee.In the middle group,there are 20 in each group.Pseudo-surgical group: exposed sciatic nerve,no treatment.Model group: After successfully molded,no processing is performed.After the successful moulding of the jump ring group and the Wenzhong group,the milli-needle acupuncture ring jump point and the Wenzhong point were used,and the electroacupuncture instrument was connected for treatment.The experimental rats were treated for 7 days and 14 days respectively.After the treatment period expires,the sciatic nerve function index of the rats after these nerve injuries is measured by means of an instrument,from which an evaluation report is made to assess the restoration of motor function in the rats after these injuries.The measurement and evaluation of the neurofunctions of these four groups of rats mainly rely on the relevant knowledge of neuroelectrophysiology.The first step is to execute experimental rats through excessive anesthesia;In the second step,the relative data of L4-L5 dorsal root ganglion were used to detect the index.The third step is to observe the pathological changes of sciatic nerve injury in rats by the staining of HE.The fourth step is to use immunohistochemical method and immunofluorescence method to detect the Schwann cells,"NCAM" and "CNTF" data in the dorsal root ganglion region of experimental rats as indicators or expression intensity;Finally,all the experimental data are taken as a statistic,and the conclusions are drawn by statistical analysis.Conclusion:1.Sciatic nerve functional index(SFI)SFI values were calculated to evaluate the functional recovery of sciatic nerve injury in rats.The SFI value of sham operation group was close to 0.On the 7th day,the SFI value of model group,circumferential jump group and central committee group decreased significantly.After acupuncture treatment,the index did not return to normal,but the SFI value was higher than that of the model group(P < 0.05),and the circumferential jump group was better than that of the central committee group(P < 0.05).It can be seen that acupuncture can promote the recovery of sciatic nerve in rats.2.Motor nerve conductive velocity(MNCV)The motor nerve conduction velocity(MNCV)showed that the MNCV of the model group was lower than that of the sham operation group(P < 0.05),indicating that the model was successful.After treatment,the loop jump group was better than the model group(P <0.05),and the loop jump group was better than the central committee group(P < 0.05).3.Observation of hematoxylin-eosin staining(HE)HE staining The morphological structure of nerve fibers in the sham-operated group was normal: the myelin sheath and axons of nerve fibers in the sham-operated group were arranged neatly and intact.It can be clearly seen that Schwann cells adhere to the edge of the rat myelin sheath nerve.The nucleus of the cells is more distinct and easy to observe.The shape of the capillary lumen is regular among these nerve fibers.In the normal control group,the nerve fibers were disordered,axons disappeared,myelin sheath disintegrated,Schwann cell apoptosis,cell nucleus blurred,and capillary lumen between nerve fibers was irregular.Compared with the model group,the degree of nerve fibers disorder was reduced,the myelin sheath structure was restored,a large number of Schwann cells were proliferated and axons were clear in the circumference of the circumferential jump group.The improvement of pathological changes in circumferential jump group was better than that in Weizhong group.4.Neurotransmission Electron Microscopy In the sham-operated group,the shape of nerve fibers was similar to that of circular cross-section.There was a tight encapsulation of myelin sheath formed by Schwann cell plasma membrane outside the axon.The axon was homogeneous,the microtubules and microfilaments(nerve filaments)were intact and abundant,the nuclear pore was not dilated,the mitochondria were arranged uniformly,and the mitochondrial cristae in the nucleus was dense and Under electron microscopy,the nuclear morphology of model group was irregular,nuclear membrane ruptured or nuclear pore dilated,endoplasmic reticulum dilated and heterochromatin condensed near the nuclear membrane,the organelles such as microfilaments and microtubules in axons were disordered,axons contracted,mitochondria swelled and deformed,and mitochondrial ridges were irregular or disappeared.The structure of nerve microfilaments,microtubules and mitochondria in the annular jumping group was clearly observed under electron microscopy,and more mitochondria were observed in the axons.The circumferential jump group was more evenly distributed than the Central Committee group,and the nerve axon developed better.5.Immune fluorescence It is known that the protein molecules of the L4-L5 ridge ganglion NCAM and CNTF of the four groups of rats are labeled with luciferin.When the corresponding antibody reaction occurs,the resulting complex has a certain amount of luciferin under the fluorescence microscope,and the fluorescence can be seen.The antigen-antibody binding site,The location of the fluorescence can also be seen.According to the location of the fluorescence display,the related properties and locations of the antibodies can be determined.After characterization,the fluorescence content in the experiment is determined by quantitative techniques.6.Immunohistochemical testing6.1 Expression of NCAM immunohistochemistry(mean optical density)in four groups of rats with L4-L5 ridge ganglion:In the four groups of experiments,NCAM proteins all had brown-yellow particles,and the measured light density was calculated:(1)Compared with the fake surgery group,the average light density of the NCAM protein in the model group was higher than that of the fake surgery group,and P <0.05,statistically significant;(2)Compared with the model group,the average optical density of NCAM protein in the loop jump group decreased compared with the model group,and P< 0.05,with a certain statistical significance;(3)Compared with the middle group,the average optical density of NCAM protein in the ring jump group decreased compared with the middle group of the higher group,and P< 0.05,with corresponding statistical significance.6.2 Expression of CNTF immunohistochemistry(average optical density)in four groups of rats with L4-L5 ridge ganglion:In the four groups of experiments,CNTF proteins all had brown-yellow particles,and the measured light density was calculated:(1)Compared with the fake surgery group,the average optical density of CNTF protein in the model group was lower than that of the fake surgery group,and P< 0.05,statistically significant;(2)Compared with the model group,the average optical density of CNTF protein in the loop jump group is lower than that of the model group,and P<0.05,statistically significant;(3)Compared with the middle group,the average light density of CNTF protein in the loop jump group was lower than that of the middle group,and P<0.05,statistically significant.6.3 Test results of Schwann cells in L4-L5 spinal ganglion in four groups of rats(average light density):In the four groups of experiments,Schwann cells all had brown-yellow particles,and the measured light density was calculated:(1)Compared with the fake surgery group,the average optical density of Schwann cells in the model group was lower than that of the fake surgery group,and P<0.05,statistically significant;(2)Compared with the model group,the average optical density of Schwann cells in the loop jump group was lower than that of the model group,and P<0.05,statistically significant;(3)Compared with the middle group,the average light density of Schwann cells in the ring jump group was lower than that of the middle group,and P<0.05,statistically significant.Results:1.Acupuncture can effectively improve the recovery of injured sciatic nerve by stabbing "Huanjuan" and "Weizhong" points of experimental rats.motor function and nerve conduction velocity in rats with acute sciatic nerve injury.The Huantiao group is superior to the Weizhong group.2.Acupuncture at "Huantiao" and "Weizhong" points can improve damaged nerve fibers,protect damaged neurons,promote axon regeneration,and promote the repair of nerve fibers.The recovery of Huantiao group is better than that of Weizhong group.3.Acupuncture at "Huantiao" and "Weizhong" points can up-regulate the expression of NCAM,CNTF and Schwann cells in L4-L5 spinal ganglion of rats with sciatic nerve injury,and the Huantiao group is better than the Weizhong group. |
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