The Effective Components Of Yixintai Regulate Ventricular Remodeling And Cardiac Muscle CaN Activity In Rabbits With Heart Failure

Objective:To explore the possible mechanism of Yi Xin Tai in treat ing HF,and to further study the mechanism of its effective components in regulating the ventricular remodeling and CaN activity in HF rabbits.Methods:1.Using HF as the disease model,the TCMSP database and its analysis platform,the HPO database,and the STRING database were used to construct the“Yi Xin Tai component-potential target-heart failure”network.The network was visualized by Cytoscape software,and then the key nodes of the network are obtained according to the node intermediary,node closeness,and node connectivity.And the gene function enrichment analysis of KEGG is performed on the key nodes with the help of the GATHER database platform to realize the network pharmacology research and predict the possible mechanism of Yi Xin Tai in treating HF.2.The models of HF rabbits were established by narrowing the abdominal aorta and giving propylthiouracil,and the rabbits successfully modeled were divided into high,middle,and low dose groups of the effective components of Yi Xin Tai,losartan potassium group and HF model group.In addition,there wa s another sham operation group.The high,middle,and low dose groups of the effective components of Yi Xin Tai and the losartan potassium group were given the corresponding drugs.The sham operation group and the HF model group were given distilled water of the same volume once a day for 4 weeks.After the experiment,the general condition,ANP,BNP,cardiac ultrasound indicators,cardiac index,leftconcentration,CaN activity in the myocardial tissue,cell apoptosis index in the myocardial were compared after each experiment.Results:1.The“Yi Xin Tai component s-potential target s-heart failure”network contains 390 nodes,131 key nodes,and 9 related KEGG pathways,which are cell cycle,focal adhesions,Toll-like receptor signaling pathway,Gap junction,Apoptosis,MAPK signaling pathway,Wnt signaling pathway,Calcium signaling pathway,Jak-STAT signaling pathway.And CaN is a common node of Wnt signaling pathway and Calcium signaling pathway.2.After 4 weeks of medication,compared with the HF model group,ANP and BNP levels in serum in each treatment group were significantly reduced?p<0.01?.Compared with the low dose group,the ANP and BNP levels in serum in the middle,high dose group s and losartan potassium group decreased significantly?p<0.01?.3.After 4 weeks of medication,compared with the HF model group,the LVEF,LVFS,and E/A of the middle,high dose group s,and the losartan potassium group were significantly increased?p<0.01?,and the IVS,LVPW,LVIDs,and LVIDd were significantly decreased.?P<0.01?.4.After 4 weeks of medication,compared with the HF model group,the heart index and left ventricular weight index of the middle,high dose group s and the losartan potassium group were significantly reduced.C ompared with the low dose group,the cardiac index and left ventricular weight index of the middle,high dose group s and the losartan potassium group decreased significantly?p<0.01?.5.After 4 weeks of medication,compared with the HF model group,the[Ca ²⁺]i concentration and CaN activity in the myocardial tissueofeachtreatmentgroupweresignificantly reduced?p<0.01?.Compared with the low dose group,the[Ca²⁺]i concentration and CaN activity in the myocardial tissue of the middle,high dose groups and the losartan potassium group decreased significantly?p<0.01?.6.After 4 weeks of medication,compared with the HF model group,the damage of myocardial cell was reduced to varying degrees in all groups,interstitial cell infiltration was reduced,edema was also reduced.Conclusion:The effective components of Yi Xin Tai can reduce the activity of CaN in myocardial tissue and improve ventricular remodeling,and the middle,high dose groups are significantly better than that in the low dose group.