Study On The Contents Of MMP-9 And TIMP-1 In Synovial Fluid Of Patients With Different Syndromes Of Knee Osteoarthritis

Objective:To observe the concentration characteristics of matrix metalloproteinase-9(MMP-9)and matrix metalloproteinase inhibitor-1(TIMP-1)in the joint fluid of patients with different syndromes of knee osteoarthritis(KOA)and their correlation with TCM syndrome score.Further explore the relationship between the severity of TCM syndrome and the index of joint fluid,try to explain the materiality basis of syndrome occurrence from a new angle,enrich the connotation of TCM syndrome differentiation,find the materiality basis of TCM syndrome differentiation,and try to provide a new idea for the clinical diagnosis and treatment of knee osteoarthritis.Methods:54 cases of knee osteoarthritis were collected by epidemiological cross-sectional investigation.15(27.8%)males and 39(72.2%)females were divided into 23 cases of phlegm stasis syndrome and 31 cases of deficiency of liver and kidney syndrome according to the criteria of TCM syndrome.Collect the X-ray of the front and side position of the knee joint of the affected side,carry out Kellgren Lawrence classification,and collect the joint fluid of the two groups of patients,use ELISA method to detect the concentration of MMP-9 and TIMP-1 in the joint fluid,analyze the difference of X-ray classification of the front and side position of the knee joint of the patients with knee osteoarthritis and liver and kidney deficiency syndrome by statistical method,the joint fluid mm of the patients with knee osteoarthritis and liver and kidney deficiency syndrome P-9,TEMP-1 concentration differences,and further analysis of knee osteoarthritis in different syndrome patients with joint fluid MMP-9,TIMP-1 concentration and TCM syndrome score correlation.Results:The age of knee osteoarthritis patients in the two groups was 27-77 years old,the mean±SD was 62.53±12.02 years old,the course of disease was 0.17-840 months,the mean ±SD was 77.16±138.21 months.There was no significant difference in gender,age,BMI,blood pressure,heart rate,respiration and the course of knee arthritis between the two groups(P>0.05)There was no significant difference between the two groups in the K-L staging of the knee joint in the anteroposterior and lateral position(P>0.05)The level of MMP-9 in the joint fluid of the patients with the syndrome of phlegm and blood stasis is higher than that of the patients with the syndrome of deficiency of liver and kidney(P<0.05),and the level of TIMP-1 in the joint fluid of the patients with the syndrome of deficiency of liver and kidney is lower than that of the patients with the syndrome ofphlegm and blood stasis(P<0.05).There was a positive correlation between TCM syndrome integral and the concentration of MMP-9(r=0.417,P<0.05),between TCM syndrome integral and TIMP-1(r=0.470,P<0.05),between,TCM syndrome integral and MMP-9(r=0.470,P<0.05)There was a positive correlation(r=0.374,P<0.05).Conclusion:1.The imaging characteristics of knee joints cannot reflect the differences in TCM syndromes of patients with knee osteoarthritis;2.The characteristics of MMP-9 and TIMP-1 concentrations in patients with knee osteoarthritis are different,indicating that the joint fluids MMP-9 and TIMP-1 in patients with knee osteoarthritis reflect the TCM syndromes to some extent.difference;3.There is a correlation between the concentrations of MMP-9 and TIMP-1 in synovial fluid of patients with knee osteoarthritis and TCM syndrome scores,indicating that the concentrations of MMP-9 and TIMP-1 in synovial fluid of patients with knee osteoarthritis can reflect Chinese medicine to a certain extent Degree of syndrome;4.The above results are consistent with TCM etiology and pathogenesis analysis.From the perspective of laboratory research,the material basis of TCM syndromes may be explained,which further enriches the internal meaning of TCM syndromes and provides objective material basis for TCM syndromes.