| Objective: To study the distribution characteristics of the mi R-107 gene rs2296616 and the mi R-210 gene rs7935908 polymorphism in healthy people in Guangxi,and to compare the distribution differences in genotype and allele frequency of the two polymorphic sites between different regions.Methods:(1)The polymorphism of the mi R-107 gene rs2296616 and the mi R-210 gene rs7935908 of the 350 healthy people from Guangxi were detected by SNa Pshot SNP typing technique and the DNA sequencing,and the distribution characteristics of genotype and allele frequency of the two loci in Guangxi population were analyzed.(2)The SNPs data of the mi R-107 gene rs2296616 and the mi R-210 gene rs7935908 in the different regions published by the Human Genome Monomer Map(Hap Map)Project were obtained from the Pub Med(SNP)database,and the differences of genotype and allele frequency of the two sites between groups of that were analyzed by statistical method.Results:(1)mi R-107 gene rs2296616 SNP contains AA(91.1%),AG(8.9%)genotypes and A(95.6%),G(4.4%)alleles in Guangxi healthy population.The frequency of genotype and allele distribution of rs2296616 were not statistically different between men and women in Guangxi population(P=0.623 and P=0.631,respectively).However,there were significant differences in the genotype and allele frequency of mi R-107 gene rs2296616 in Guangxi healthy population compared with those of Europeans,Japanese,Africans,Mexicans and Indians published in Hap Map(P<0.05),but was not statistically significant difference compared with the Han population in Beijing(P=0.900 and P=0.902,respectively).(2)mi R-210 gene rs7935908 SNP contains AA,AG and GG genotypes in Guangxi healthy population,the frequencies of which were 45.4%,44.6% and 10.0%,respectively.The allele frequencies of A and G were 67.7% and 32.3%,respectively.The frequencies of genotype and allele distribution of rs7935908 were not significantly different between genders in the Guangxi population(P=0.383 and P=0.763,respectively).However,there were significant differences in the genotype frequency of mi R-210 gene rs7935908 in Guangxi healthy population compared with those of Europeans,Han Chinese,Japanese,Africans,Mexicans and Italians published in Hap Map(P=0.042,P=0.004,P=0.018,P<0.001,P<0.001 and P=0.005,respectively),but was not statistically significant differences compared with Hap Map-CHD(P=0.743).The allele frequencies of healthy population in Guangxi were significantly different from those of Europeans and Africans,Mexicans and Italians(P=0.028,P<0.001,P<0.001 and P=0.002,respectively),but were not significant differences compared with Han Chinese,Hap Map-CHD and Japanese(P=0.418,P=0.458 and P=0.777,respectively).Conclusion:(1)there existed mi R-107 gene rs2296616 and mi R-210 gene rs7935908 genetic polymorphism in Guangxi healthy population.(2)The distributions of genotype and allele frequency of mi R-107 gene rs2296616 and mi R-210 gene rs7935908 are not statistically significant among different genders in Guangxi healthy population.(3)The distributions of mi R-107 gene rs2296616 and mi R-210 gene rs7935908 polymorphism in Guangxi healthy population are different degrees of discrepancy from that of other regional groups.Objective:(1)To investigate the association between mi R-107 gene rs2296616 and mi R-210 gene rs7935908 polymorphisms and ischemic stroke.(2)To detect the expression of mi R-107 gene and mi R-210 gene in peripheral blood mononuclear cells of ischemic stroke patients and healthy controls,and to analyze the correlation between rs2296616 and rs7935908 polymorphisms and the expression of mi R-107 and mi R-210 genes.Methods:(1)The genotypes of the mi R-107 gene rs2296616 and mi R-210 gene rs7935908 polymorphisms of the 349 ischemic stroke patients and 372 healthy controls were detected by SNa Pshot SNP genotyping assay and DNA sequencing.(2)Applied Biosystems 7500 Real Time PCR System was used to detect the expression of mi R-107 gene and mi R-210 gene in peripheral blood mononuclear cells of ischemic stroke patients and healthy controls.(3)The serum lipid-related indexes of ischemic stroke patients and healthy controls were detected by Hitachi automatic biochemical analyzer 7600.Results:(1)The levels of the serum total cholesterol(TCH),low-density lipoprotein cholesterol(LDL-C),extremely low-density lipoprotein cholesterol(VLDL-C)and triglyceride(TG)in ischemic stroke group were significantly higher than those in control group,while high-density lipoprotein cholesterol(HDL-C)level was significantly lower than those in the control group(P<0.05).(2)There were AA,AG and GG genotypes of mi R-107 gene rs2296616 in the ischemic stroke group and AA and AG genotypes in the healthy control group.The mi R-210 gene rs7935908 polymorphism includes AA,AG and GG genotypes in both the ischemic stroke group and the healthy control group.Moreover,the frequency distribution of the two polymorphic loci in the ischemic stroke group and the healthy control group were all in accordance with the genetic balance of Hardy-Weinberg(P >0.05),which had obvious population representativeness.(3)There was no significant difference in the frequency distribution of genotype,allele,dominant model(GG+AG vs.AA),recessive model(GG vs.AA+AG)and hyper-dominant model(GG+AA vs.AG)between ischemic stroke group and healthy control group,regardless of whether or not the confounding factors were corrected(P>0.05).The results of stratified analysis also showed that rs2296616 and rs7935908 polymorphisms were not statistically different in the stratification of age,sex,hypertension history and diabetes history in the ischemic stroke group(P>0.05).rs2296616 and rs7935908 polymorphisms were also not associated with serum levels of TCH,HDL-C,LDL-C,VLDL-C and TG.(4)The expressions of mi R-107 and mi R-210 in peripheral blood mononuclear cells in ischemic stroke group were significantly higher than that in healthy control group(P<0.001),but the abnormal expressions of mi R-107 and mi R-210 were not correlated with the polymorphism of rs2296616 and rs7935908 locus(P>0.05).Conclusion:(1)The mi R-107 gene rs2296616 and the mi R-210 gene rs7935908 polymorphisms were not associated with the occurrence of ischemic stroke.(2)The expression of mi R-107 and mi R-210 in peripheral blood mononuclear cells in the ischemic stroke group was significantly higher than that in the control group,both of which may be potential novel biomarkers for early diagnosis of ischemic stroke.(3)The polymorphism of rs2296616 and rs7935908 did not regulate the expression of mi R-107 and mi R-210 in ischemic stroke. |
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