| Due to the range of species affected, the high rate of infectivity, and the fact that virus is shed before clinical signs occur, foot-and-mouth disease (FMD) is one of the most feared reportable animal disease in North America.; Herein we describe the development and characterization of 3 murine monoclonal antibodies F24G1, F24G2 and F24G3 to the VP1 protein of the FMD virus. The mice underwent a complex sequential immunization strategy designed to select for B cells reactive against epitopes conserved between different serotypes. While our data show that these mAbs recognize type A24Cruzeiro and Asia I FMD virus specifically in ELISA using whole virus, the mAbs neutralize the infectivity of only serotype A24 in vitro in cell culture. All 3 mAbs recognize trypsin-sensitive, linear, heat-stable epitope(s). We have determined the molecular sequence of the immunoglobulin variable regions of these hybridomas in order to analyze clonal relatedness of the B cells from which they were derived. (Abstract shortened by UMI.)... |
