| T cell signaling pathway involves complicated intermolecular and intramolecular interactions. Interleukin-2 tyrosine kinase (Itk), cyclophilin A (CypA) and hematopoietic lineage cell-specific protein (HS1) are three key mediators in this pathway. This thesis explores the negative regulation of Itk activity by CypA and the autoinhibitory regulation mechanism by the HS1 protein through biochemical and structural methods. |
