Metal-Responsive Proteomes: Copper, Iron, Zinc, and Manganese Micronutrient Deficiency in Chlamydomonas reinhardtii

Trace metals like copper, iron, zinc and manganese play important roles in several biochemical processes including respiration and photosynthesis. Using MSE proteomics data acquisition techniques I examined the effects of deficiencies of these micronutrients on the soluble proteome of Chlamydornonas reinhardtii. I measured the abundance of more than 1,000 proteins with a dynamic range of 3-4 orders of magnitude and demonstrated statistically significant changes in over 200 proteins in each metal deficient growth condition relative to cells grown in nutrient replete media. I describe the individual and inter-relational impacts of each of metals on Chlamydomonas biochemistry. I also examined the correlation between protein and transcript levels and found moderate correlations with respect to abundance, which increased for those situations where transcript abundances increased dramatically.