| Chronic diabetic wounds are a serious complication of diabetes mellitus, representing more than 27% of total annual diabetic health care costs which exceed ;The long-term goal of this research is to develop a therapy to create a wound microenvironment which enhances neovascularization and overall healing in diabetic wounds by utilizing peptide nanofibers. The studies in this dissertation contribute to this goal by investigating the nature of interactions between ECs and nanofibers, as well as the role of bone marrow-derived EPCs during nanofiber-mediated neovascularization. The central hypothesis of this research is that the RAD16-II peptide nanofibers enhance wound neovascularization and improve diabetic wound healing by regulating both angiogenesis (by ECs) and vasculogenesis (by EPCs).;The results from this dissertation research help identify the roles of ECs and EPCs during nanofiber-mediated neovascularization. The results from this study demonstrate that the nanofibers promote wound neovascularization mostly by increasing EC recruitment via an integrin dependent mechanism, with a limited contribution from bone marrow-derived EPCs. The findings of this study will contribute towards developing an optimal microenvironment to enhance diabetic wound neovascularization and for vascular tissue engineering applications. |
