Development of Trace Analysis Methods by Spectrofluorimetry

An investigation of light scattering of non-imprinted PMAA-co-EGDMA particles (NIP) in aqueous suspension after 17beta-estradiol (E2) binding was conducted by spectrofluorimetry. This light scattering from NIP particles in aqueous suspension was increased by spiking E2 from 0.1 to 1.0 ppm. Thus, a new alternative method was found to determine 17beta-estradiol (E2) by measurement of light scattering from NIP particles.;Molecularly imprinted PMAA-co-EGDMA particles (MIP) were synthesized in the presence of template E2 molecules. Fluorescence emission from E2 non-specifically bound onto the MIP particles was quenched by gold nanoparticles. The S-V plot was linear, with Ksv=2.9x104 M-1. Fluorescence emission from E2 specifically bound inside the MIP particles was quenched by nitrite anions. The S-V plot became non-linear, with K sv=2.1x102 M-1 and V<1.0 M -1. The difference between these two emission intensities varied with the initial E2 concentration in water, generating a Scatchard calibration curve from 0.1 to 10 ppb.