| Two stationary phases were tested for retention of polynuclear aromatic hydrocarbons on high-performance liquid chromatography. A bonded nematic liquid crystal stationary phase was utilized, containing the silanized form of 4- (4-(allyloxy)benzoyloxy)biphenyl. A polymeric stationary phase, Vydac 201TP C{dollar}sb{lcub}18{rcub}{dollar}, was also examined. Both were tested over the temperature range of 25{dollar}spcirc{dollar}C to 70{dollar}spcirc{dollar}C, using anthracene and phenanthrene. As the temperature of the stationary phase increased, a decrease in retention was observed, as expected. Both phases showed shape selectivity, supporting the slot theory of retention. Retention based on the planarity of the solute was observed by the polymeric phase, owing to the ordered state of the bonded phase. Mobile phase composition was also varied on the Vydac column. Polarity did exhibit an effect on the retention of anthracene and phenanthrene, the more polar solvent resulting in longer retention times. |
