| he growth of Escherichia coli is only slightly inhibited when the cells are incubated with 20 ;Much higher DBP concentrations are needed to block the growth of a pgsA3 mutant than to block the growth of an isogenic wild-type strain. The DBP-treated pgsA mutant also has a much higher survival rate when stored in the cold than does the DBP-treated wild-type strain. Furthermore, the pgsA3 mutant grows normally in the presence of DBP and magnesium chloride. Treatment with DBP and magnesium ions does not appear to disrupt the cell's inner or outer membranes. The combination of DBP and magnesium chloride does not have serious metabolic consequences for the pgsA3 mutant strain but inhibits macromolecular, phosphoglyceride and ATP synthesis in wild-type strains. A combination of 20... |
