PHYSIOLOGICAL ASPECTS OF THE ACETONE-BUTANOL FERMENTATION

The effect of the key physiological parameters on the production of solvents in the acetone-butanol fermentation using the anaerobic bacterium Clostridium acetobutylicum was examined in this work.;A linear correlation was established between the production of solvents and gases which varied with the mixing rate of the fermentation system.;Elevated hydrogen partial pressure affected the metabolism of C. acetobutylicum resulting in increased butanol and ethanol yields (based on glucose) by an average of 18% and 13%, respectively.;A mathematical model for the batch acetone-butanol fermentation was formulated using original experimental data for the microbial growth, sugar consumption and metabolite biosynthesis. This model was used for computer process simulations. Parametric sensitivity analysis indicated the importance of the key process parameters.;The theoretical solvent yield was calculated based on expressing stoichiometric relationships between the substrate and the products of the process. The maximum theoretical yield under the acceptable process conditions was established ranging from 38.6% to 39.9%.;A method of systems analysis was applied in analysing pronounced physiological differences in the performance of one of the C. acetobutylicum culture strains. The cellular transport mechanism for substrate (glucose), solvents and acids through the cell membrane was established to depend on its permeability and the number of sugar transport "sites". Experimental results obtained from the study of the uptake of 3-0-methyl glucose (0.7mM) by the "normal culture" and the "retarded culture" confirmed the theoretical predictions of a slower transpost in the "retarded culture". The theoretical predictions were further confirmed by additional experimental results.;A mathematical "Physiological State Model" was developed which includes the culture physiological parameters as well as the internal and the external culture conditions. Using this mathematical model the standard and the substandard acetone-butanol fermentations could be simulated.;These results demonstrate the application of the method of systems analysis in elucidation of the role played by the key culture physiological parameters in the fermentation process.