Structure And Functional Potential Of The Inter-Root Soil Microbial Community Of Plants In The Shoreline Zone Of Daihai Lake

Inter-rooted soil microorganisms are an organism group with a high abundance of species community structure in the earth’s ecosystems.Studies have shown that the community structure and diversity of plant inter-rooted microorganisms are influenced by different plants,soils,plant inter-rooted microorganisms and other organisms such as protozoa,which are involved in the transformation of all soil materials and have an important impact on the ecosystem.The Daihai watershed is an important part of the ecological barrier in the BeijingTianjin-Hebei region.In recent years,as the water volume in Daihai has decreased,the salinisation of the water body has intensified,the total mineralisation has risen,and the exposed mudflats as well as the lakeshore zone have become more sandy and saline under wind transport,and soil erosion has increased.In this highly saline environment,the interrooted soil microbial community of plants in the lakeshore zone must be characterised by special features.In this study,the inter-rooted soil microorganisms of alkali poncho,cranberry,Mitsubishi water onion,balsamroot and reed in the shoreline zone of Daihai Lake were selected for the study,and the structural and functional diversity of the inter-rooted soil microbial community of each plant type was examined by macro-genetic sequencing technology.The relationships between the structure and function of inter-root soil microbial communities and environmental factors such as soil environmental factors(p H,total nitrogen,total salinity,total phosphorus)and water environmental factors(TN,TP,p H,CODMn,F-,T)were analysed,and the main findings were as follows.(1)The overall soil of the shore zone of Daihai Lake is sandy and saline due to lake degradation,low precipitation and high evaporation,among which the salinization of MT and DC soils is the most serious,with the total salt amount reaching 2.01% and 2.58%respectively.The p H of the soil is alkaline,and the p H of MT and DC has even reached a strong alkalinity.The situation of the water bodies in Daihai is also not optimistic,with TN far above the standard for surface V water and fluoride concentrations remaining high,exceeding surface V water by 2.7 times.Overall,it seems that the water quality at MT,DC and GB is poor,and the plant growth environment is relatively poor.(2)After preliminary processing of the samples by macro-genome sequencing,there are five species of Domain,eight species of Kingdom,129 species of Phylum,262 species of Class,583 species of Order,1087 species of Family,2893 species of Genus and 14,399 species of Species.14,399 species.The phylum Green Bacteria has a high abundance at all sampling sites,while the phylum Thick-walled Bacteria has a very high abundance at SDH,and the phylum Acidobacteria has a definite abundance at DC compared to the other sampling sites.Comparison with the NR database shows that Acidobacteria,Chloroflexi,Proteobacteria and Actinobacteria are the dominant populations at the phylum level,while Chloroflexi,Acidobacteria,IIumatobacter and Acinetobacter are the dominant populations at the genus level.Acidobacteria,IIumatobacter and Acinetobacter were the dominant populations at the phylum level.Combined with the PCo A principal coordinates analysis and sample level clustering analysis,it can be seen that the three plant inter-rhizosphere microorganisms DC,MT and GB are more similar at different levels of classification,while geographical and environmental factors have a greater influence on them,and the above three sites have more similar microbial types due to severe soil salinization.(3)Comparison of gene sequences with the COG database showed that the relative abundance of E: amino acid transport and metabolism and C: energy production and conversion functions accounted for a relatively high proportion of soil microorganisms at SDH and TC plants inter-root,reaching 9.6%,9.7% and 9.9% and 10%,respectively.In contrast,P: inorganic ion transport and metabolism accounted for a higher abundance of6% at DC and MT plants,and the abundance of this function was directly affected by the severe salinization of the soil and poor water environment at the three sampling sites of DC,GB and MT.(4)According to the correlation Heatmap plots: TN,p H and SAL are the main influencing factors of inter-root soil microorganisms of Daihai plants.The correlation between environmental factors and Function function levels showed that E: amino acid transport and metabolism,C: energy production and conversion,P: inorganic ion transport and metabolism,L: replication,recombination and repair were significantly correlated with p H,TP and SAL.p H was a key factor affecting the functional potential of inter-rhizosphere soil microorganisms of plants in the lakeshore zone.The study of the characteristics and functions of different types of plant-microbial communities in the lakeshore zone in a high-salt environment is of great significance to the environmental management and ecological research of the Daihai Sea.By carrying out research on the ecological environment of the lakeshore zone,it can effectively promote the rate of development of the ecological environment of the lakeshore zone towards a benign and healthy development and accelerate the formation of effective protection for the Daihai Sea,which is an important way to promote the ecological recovery and health of the Daihai Sea.