Synthesis Of Small Molecule Py-BODIPY Fluorescent Probe And Its Application In Super-resolution Imaging Of Live Cell Mitochondria

As the "power factory" of eukaryotic cells,mitochondria can produce ATP through glycolysis,oxidative phosphorylation and other ways to provide energy for cell life activities.Mitochondria are usually rod-shaped or spherical,but can also be dumbbell-shaped,flat-shaped or other shapes under different species and physiological conditions.If the number,size and structure of mitochondria are changed,their function will be abnormal,which will lead to cell pathology and even body diseases.Therefore,mitochondria play a vital role in the organism.Over the years,fluorescence imaging technology has been widely used in the study of the structure and function of cells and their sub-cells.Due to the optical diffraction limit and the lack of fluorescent probes that have excellent photophysical properties and specifically target mitochondria,commercially available mitochondrial commercial probes and ordinary fluorescence microscopes cannot track and observe the 3D dynamic changes of mitochondria and their ultrastructure for a long time.process.In this article,we designed and synthesized a small molecule fluorescent probe with the characteristics of high quantum yield,strong photostability,long fluorescence lifetime and mitochondrial targeting specificity with BODIPY as the fluorescent mother nucleus,combined with STED super-resolution Fluorescence microscope clearly observes the ultrastructure of mitochondrial ridges in living cells,realizing3D-STED real-time imaging of the dynamic changes of mitochondria and their ridges during cell apoptosis.The specific research content is as follows:1.We use N-B-N BODIPY as the backbone to reasonably construct a new pyridineBODIPY derivative,namely Py-BODIPY.Because the fluorine(F)connected with BODIPY has a strong electron absorption capacity,the electron cloud density in the Py-BODIPY molecule is low,which is beneficial to specifically target the mitochondria with high membrane potential(MMP,??m),and contains nitrogen five.The membered heterocyclic ring contains 4methyl groups to increase its lipophilicity,so it can specifically bind to the mitochondrial membrane.2.The study of photophysical properties found that Py-BODIPY has ultra-high quantum yield,longer fluorescence lifetime,and fluorescence "Turn-ON" effect.Cell experiment studies have shown that Py-BODIPY has low cytotoxicity,excellent biocompatibility,ultra-high photostability and can be used for long-term super-resolution imaging of mitochondrial ultrastructure and realizes 3D-mitochondrial ultrastructure.STED image construction.3.Based on the fluorescent probe Py-BODIPY has excellent properties in super-resolution imaging,so we continue to explore the real-time 3D dynamic changes of mitochondrial ultrastructure during cell apoptosis,and count the structure of mitochondria and their cristae Chemical treatment provides a new method for the research of mitochondrial-related diseases.