Application Of Iridium Complex Bound To Dna In Cell Imaging

The nucleus is the center of the cell and the center of genetic information,the storage of which in all eukaryotes is almost entirely encoded in the biopolymer deoxyribonucleic acid(DNA).The visualization of the nucleus provides information about DNA replication,gene regulation,DNA damage/repair and other processes,as well as drug targets for the treatment of human diseases.Fluorescence imaging technology has many advantages,such as high spatial resolution and sensitivity,small damage to biological samples,simple preparation and easy operation.It occupies an important position in the field of biomedical science and is widely used in imaging and detection.Our team synthesized a water-soluble iridium complex.Due to its excellent luminescence properties and strong photobleaching resistance,we applied it to superresolution imaging and explored its biological application prospects.The probe was found to be a DNA photoswitched probe in the biological specific response experiments at the solution level of biomacromolecules.Preliminary imaging experiments at a variety of cell levels showed that the probe was localized to the nucleus and had specific recognition of DNA in the nucleus.Subsequent toxicity experiment,the positioning experiment,light bleaching experiment results further show that the probe light such as low toxicity,low noise and long life of fluorescent,strong resistance to photobleaching advantages,we use the probe with different nuclear DNA in the cell cycle of super resolution imaging and the nucleus of nanoscale structure information(below 30 nm);Due to the high electron cloud density,the metal complexes also exhibit good contrast in TEM imaging,and can be used as nuclear DNA contrast agents in TEM.In addition,the probe has the properties of two-photon fluorescence emission,so it has little damage to biological samples and strong penetration.Therefore,it has strong advantages in depth development in vivo imaging of Caenorhabditis elegans and zebrafish.Therefore,the complex shows great potential to provide information related to the structure of nuclear DNA in future studies.