Study On Culture-free And Culturable Bacterial Diversity In Three Pacific Abyssal Sediments

With the increasing consumption of land resources and the continuous progress of science and technology,it is more urgent to explore and research new resources.The deep-sea system is an important link in the whole earth ecology,with abundant resources and extreme environments different from those on land.Due to its very special ecological conditions,the microbes here have unique values different from those on land.Therefore,the development and utilization of deep-sea resources have been paid increasing attention by researchers at home and abroad and become an important research field of Marine science.The study on the diversity of deep-sea bacteria can not only enrich the diversity of existing bacteria,but also enable people to have a deeper understanding of the functional value of deep-sea bacteria,laying a foundation for the future research on the recycling of deep-sea bacteria.In this paper,the deep-sea bacteria from three deep-sea sediment samples from the eastern and western Pacific oceans were cultured in different media,and their species resources were obtained through isolation and purification.The 16 S r DNA sequence analysis was used to preliminarily identify and analyze the diversity of some isolated and cultured strains.To investigate the effect of isolated strains on the growth of an aflatoxin producing bacterium(aspergillus parasiticus).At the same time,the diversity of no-culture bacteria in three sediment samples was studied by bioinformatics analysis.By analyzing the diversity of bacteria cultured in BC13,BC14,and BC26 samples,it can be concluded that Alcanivorax Jadensis and Marinobacter Algicola are common dominant species.A total of 544 strains of deep-sea bacteria in BC13,BC14,and BC26 samples were isolated and cultured using 20 culture media of 5 basic types.Some strains were sequenced to obtain 89 identification results,which were divided into 29 genera in total.There are a large number of strains of Marinobacter,Erythrobacter,Aurantimonas,and Alcanivorax.All the 544 strains isolated were screened against parasitic aspergillus strains,and it was finally found that there were 65 active deep-sea bacteria that showed significant inhibition to the mutant strain NFRI-95 of parasitic aspergillus,among which a total of 20 strains had a red inhibition rate of up to 100%,which has a very high follow-up research value.Through the study on the culture-free and culturable bacterial diversity of three deep-sea sediment samples from the Pacific Ocean,the composition of deep-sea bacterial community,the characteristics of species and gene composition,and the specificity of bacteria in deep-sea environment are understood,which will provide important information for the study on the functionalization of bacteria in deep-sea sediments.