Construction And Application Research Of High Specific Virus Molecular Imprinted Photochemical Sensor

In recent years,the world has entered a period of frequent virus outbreaks,such as the continuous outbreak of COVID-19.Virus analysis and selective detection is a continuous development field.Therefore,the highly sensitive detection and specific recognition of viruses have attracted extensive attention.Inspired by the process of biological discovery,such as enzyme substrate and antibody antigen interaction,virus molecular imprinting sensing technology is an effective method for specific identification of viruses developed in recent 15 years.However,due to the complexity of virus size,surface structure and the similarity between different subspecies,the specific recognition ability of molecular imprinting technology for virus is far from small molecule level.Therefore,it is a great challenge to improve the biocompatibility,specificity and sensitivity of virus molecular imprinting biosensor.Therefore,we need to break through the inherent related viral imprinting strategies and the key obstacles in the synthesis of viral imprinting materials.Therefore,we have constructed two kinds of virus molecular imprinting biosensors to solve the above problems.The main research work was as follows:1.A gas responsive virus imprinting biosensor based on zeolites imidazolium ester(ZIF-8)was designed.Using ZIF-8 as carrier,dimethylaminoethyl methacrylate as gas responsive functional monomer,acrylamide as comonomer,ammonium persulfate and sodium bisulfite as initiator,the Viral imprinting polymers were prepared.The gas sensitive imprinted polymer designed by this strategy not only improves the specific recognition ability of the sensor to the template virus,but also effectively avoids the response failure caused by substance accumulation caused by stimulation compared with other stimulation imprinted polymers.The experimental results showed that the sensor has good selectivity for template molecules,the imprinting factor(IF)was as high as 6.7,and the detection limit was as low as 9.1pm.This strategy provides theoretical support for the application of molecular imprinting technology in the clinical diagnosis of virus,and has important application prospects.2.Two kinds of HAV-nanoMIP and HBV-nanoMIPs nanoparticles were synthesized by template modification of solid carrier materials based on magnetic microspheres.The nanoparticles can adhere to the surface of carrier materials.After synthesis,the particles can be purified by in situ affinity to produce two template free forms of high affinity receptors.In addition,two different dyes were synthesized as the output source of different signals,which can realize the simultaneous detection of two kinds of hepatitis virus.Finally,this work achieved satisfactory selectivity,and the selection factors were all greater than 5,which was higher than that of previous reports.Moreover,the template removal and binding test do not need to repeat the lengthy steps such as washing/centrifugation or dialysis.This is of great significance to solve the problem of insufficient affinity of traditional detection methods for macromolecular targets.