Preparation,Characterization Of Magnetic Cross-linked Starch Composite And Studies Of Its Adsorption For Lysozyme

Combined method was used to prepared cross-linked starch by taking Cassava starch as raw material.Cross-linked starch was mixed with magnetic material to get magnetic cross-linked starch composite.The product was used in lysozyme adsorption,the adsorption dynamics behavior,Isothermal adsorption,thermodynamics were studied.Cassava starch and cross-linking agent sodium hexametaphosphate were taken as raw materials which reacted under the synergistic effect of ultrasonic and microwave.Orthogonal experiment(L₁₆ 4⁵)was designed based on single factor experiment.Cross-linked starch prepared in the optimum conditions was characterized by Fourier Transform Infrared Spectroscopy(FT-IR),X-Ray Diffraction(XRD),Field emission scanning electron microscopy(FESEM).Self-made cross-linked starch was taken as reactant for the next step,mixed with inorganic magnetic material Fe₃O₄,initiator ammonium persulfate and cross linking agent glutaraldehyde were added by order to react.The product was in spray drying to get magnetic cross-linked starch composite.Response surface test of five factors three levels was designed in considering of single factor experiment to get optimum conditions.Production prepared in the optimum conditions was measured by FT-IR,XRD,TG-DSC,and VSM.The self-made magnetic cross-linked starch composite material was used in lysozyme adsorption study to explore the influence of adsorbent dosage,p H value,and adsorption temperature,adsorption time on the adsorption rate.And the adsorption dynamics behavior,Isothermal adsorption,thermodynamics were studied.According to the dynamic model parameters,adsorption process is spontaneous,endothermic reaction and pushed on by entropy.