Interface Fabrication Of Functional Carbon Nanomaterials And Their Applications For Recognition And Therapy

Carbon nanomaterials exhibited excellent physical and chemical properties,due to a small size effect and surface effect.They attracted scientists' attention because having great potential applications in the fields of tumor therapy,gene/drug carrier,optoelectronic devices,sensing and so on.In this paper,we focused on the research of fabrication of functional nanomaterials,and the properties were also investigated.The main works were summarized as follows:(1)General classification,properties,synthesis,functionalization and characterization methods of carbon nanomaterials,as well as their applications in various fields,such as sensing,optoelectronic devices and biological therapy,are described in this chapter,respectively.Moreover,the research mentality and contents of this article are proposed as well.(2)A kind of hydrophobic carbon nanodots with white fluorescent emission,white fluorescent carbon nanodots(WCDs)were prepared by carbonizing tween 80 with sulfuric acid and phosphoric acid through one-step method.The results of HRTEM,NMR,Raman spectroscopy,X-ray photoelectron spectroscopy and Infrared spectroscopy illustrated that as prepared WCDs were spherical nanoparticles with hydrophobic alkyl chains on the surface.Fluorescence spectroscopy showed WCDs have a strong fluorescence emission in both aqueous and solid state.In addition,the Commission Internationale de L'Eclairage(CIE)1931 chromaticity coordinate for WCDs in solid state is located(0.270,0.306),which is close to pure white light(0.33,0.33).The fluorescent quantum yield data indicated that the alkyl chain on the surface of WCDs make them effectively suppress the aggregation-induced quenching(AIQ)effect.The prepared WCDs were applied for white light emitting diode(W-LED)and the recovery of fingerprints.CIE 1931 chromaticity coordinate for W-LED device is located(0.310,0.296)with a CCT of 7034 K,which belongs to a cold white light.Moreover,clear and bright fingerprint image can be seen under UV light,which explained that WCDs nanoparticles can dissolve with lipophilic substances in sweat well.(3)Carbon nanomaterials have good photoelectric conversion performance,but hard to dissolve in the solution,which is an emergency problem for their application in the field of biological applications.Hypericin,a kind of natural photodynamic agent,is extracted from the forsythia plant.We presented a new and facile one-step method for the fabrication of a water-soluble SWNHs-Hypericin nanohybrid material by simply sonicating Hypericin and SWNHs.The obtained SWNHs-Hypericin nanohybrids were applied for photothermal therapy(PTT)and photodynamic therapy(PDT)to kill tumor cells.In this PTT/PDT nanosystem,SWNHs acts as a carrier and PTT agent,while Hypericin acts as a hydrophilic and PDT agent.Hypericin was coated on the surface of SWNHs via non-covalently ?-? interaction,detailedly characterized by HRTEM,UV-vis spectroscopy,raman spectroscopy and electron paramagnetic resonance spectroscopy.An electron transfer phenomenon was appeared between Hypericin and SWNHs under light irradiation result in generating reactive oxygen species.The test of photothermal conversion proved that SWNHs-Hypericin nanohybrid can caused temperature increased significantly under laser irradiation in vitro and in vivo.Moreover,the results of cell viability revealed SWNHs-Hypericin nanohybrid have a good light treatment effect in vitro.Animal experimental results showed that SWNHs-Hypericin nanohybrid can kill breast cancer cells effectively,having a good anti-tumor activity.Undoubtedly,SWNHs-Hypericin nanohybrid with PTT/PDT effects is a hopeful candidate material for biotherapy application.