Study On Subtraction Technique Of Carbamoyl Compounds In Rice Wine

Rice wine will produce carcinogenic compounds ethyl carbamate(EC)in the brewing process.Studies have shown that ethyl carbamate in yellow rice wine is mainly produced by the reaction of ammonia formyl compounds and ethanol in wine.The deduction of carbamyl compounds in wine can improve the security of wine.So the study of deduction of carbamoyl compound in yellow wine is very meaningful.The main research contents of this thesis are as follows:1.The research used chitosan as the matrix material,used sodium hydroxide as the coagulating reagent and used glutaraldehyde as crosslinking agent to prepared carrier microspheres of immobilized enzyme.The chitosan microspheres were used as a carrier to immobilize urease and prepare the deduction agent of carbamyl compounds.The enzymatic properties of the deduction agent of carbamoyl compound were compared with that of free urease.The results showed that the deduction agent of carbamoyl compound have the larger p H and temperature tolerance range and the better reusability and storability.2.The deduction agent of carbamoyl compound was used to carry out the reduction of carbamoyl compound in yellow wine.It can be seen in single factor experiment that the adding amount of deduction agent,reaction temperature and reaction time are the main factors of the reduction of carbamoyl compound in yellow wine.Based on the three factors,the orthogonal test was carried out and the best treatment process was obtained that the add amount of deduction agent is 1.5 %,the treatment temperature is 35?,and the processing time is 2 h.Under this condition,the deduction rate of urea can reach 43.96%.3.The deduction agent was added in the period of after-fermentation and before fried wine to reduce carbamoyl compound in yellow rice wine.The results showed that the deduction agent have the effect on reduction of carbamoyl compound and it have no obvious effect on alcohol content,total acid and total sugar during fermentation period of yellow rice wine.When the deduction agent was added before fried wine,the deduction rate of urea is 43.81% and the deduction rate of EC is 34.45% which indicated that added deduction agent before fried wine could effectively reduce the EC in the period of fried wine.4.Utilizing chromatography column,the unit device of removing carbamoyl compounds in yellow rice wine was prepared.The processing parameter of device of removing carbamoyl compounds was investigated on a laboratory scale.Cascading unit device,the device of removing carbamoyl compounds in yellow rice wine was built and the process flowsheet of it used in actual production was designed,which established the foundation of applied the device of removing carbamoyl compounds to actual production of yellow rice wine.The deduction agent of carbamyl compounds was successfully prepared.It can efficiently reduce the content of urea in yellow rice wine and can be reused.It effectively inhibited the generation amount of ethyl carbamate from the source and improved the security of yellow rice wine.The obtained results have important reference value for practical application of removing carbamoyl compound.