Preparation Of Photolithography Hydrogel Particles And Its Application In Intelligent Detection Of Food Toxins

Mycotoxins were one of the most common and harmful food safety risk factors.However,mycotoxins coexist normally in the same food and could produce synergistic or antagonistic effects.So,it's difficult to achieve with multiple tests and high throughput.Therefore,it is important to develop a portable and rapid method for detecting a variety of coexisting mycotoxins.Here,we built a smartphone-based mycotoxin detection system,which combines shape-coded hydrogel particle preparation and image processing technology in smartphones together.First,shape-coded hydrogel microparticles containing specific recognition aptamers were synthesized by stop flow lithography.Second,aflatoxin B1 and ochratoxin A were selected as analytes,and the use of hydrogel for detecting mycotoxins was investigated using aptamer recognition technology.Third,capture images with cameras on the smartphones.Then the app analyzes the images and delivers the results.Fourth,an ultrafast laser system has been built and its biology benefits were verified.Multiphoton lithography has been studied prospectively.The conclusions of the experimental research make it possible to draw the following conclusions: first,the hydrogel particles produced are intrinsically stable and the coding signal is clear.The aptamer-based detection method is simple and fast,the total reaction time does not exceed 1 h and 45 min and the limit of detection is 0.1 ng/m L.It can be seen that the method has a large detection range and a high sensitivity.Secondly,the test results can be excited from a simple portable device and captured by the smartphone camera.Then the image is analyzed and the result is obtained from the app.Finally,the ultrafast laser was used to replace the ultraviolet light source used in conventional lithography,and the PEG hydrogel material was successfully solidified to confirm the possibility of multiphoton lithography.In summary,this article provides a fast,high-speed portable detection solution for real-time and off-site detection of mycotoxins.This smartphone-based platform is expected to realize its benefits in a variety of areas,such as food safety,environmental monitoring and disease diagnosis,and will create more application channels.