Study Of Fluoride On The Changes Of Oral And Intestinal Microorganisms And Intestinal Morphology

Fluorine is one of the essential tr ACE elements in the human body.Proper amount of fluoride can prevent dental caries and promote the formation of strong bones,and excessive intake of fluoride for a long time may cause fluoride poisoning.At present,the effect of different doses of fluorine on intestinal flora is still unclear.In this experiment,in vitro fermentation was used to study the effects of different concentrations of fluoride(1,2,10,15 mg/L)on the intestinal flora.The established rat model of dental fluorosis was used to explore the oral cavity and intestine in the case of fluoride poisoning.Changes in microorganisms.The main research contents are as follows:1.In vitro fermentation was used to investigate the effects of different concentrations of fluoride(1,2,10,15 mg/L)on intestinal flora.The results show that:(1)Low-dose fluorine(1 and 2 mg/L)has limited influence on the structure and functional KEGG pathway of intestinal flora.However,low-dose fluorine can promote the growth of beneficial intestinal microflora,including Freundella and lactic acid bacteria.(2)High-dose fluorine(10 and 15 mg/L)significantly changed the composition and functional KEGG pathway of intestinal flora.At the same time,high-dose fluorine reduces the beneficial intestinal flora(Fischerella and Koala bacteria);increases harmful bacteria(Proteus and Enterobacter).(3)Different doses of fluorine have limited effects on short-chain fatty acids(SCFAs).The results show that different doses of fluorine have different effects on the intestinal flora.2.Using the established rat model of dental fluorosis,explore the changes of microbial flora in the oral cavity and intestine in the case of fluorine poisoning.The results show that:(1)The diversity and richness of oral microorganisms will decrease under the condition of fluorosis,but the impact on the KEGG pathway is limited.During fluorosis,the oral flora is disturbed,the relative abundance of harmful streptococcus and staphylococcus increases,and the relative abundance of beneficial bifidobacteria decreases.(2)Fluorine changes the composition of intestinal microbes.The intestinal microbial diversity and abundance in the high fluoride treatment(HF)group was slightly higher than that in the blank(C)group,but there was no significant difference.The abnormal increase of Lactobacillus in the high-fluoride group may be due to the intestinal damage caused by the disturbance of intestinal homeostasis.According to the function prediction of the intestinal flora of rats through KEGG,fluorine poisoning will reduce the energy metabolism,vitamin metabolism,amino acid metabolism and immune system function of rats,which is manifested in the significant reduction in the weight of mice.(3)The intestinal damage is serious during fluorosis,a large amount of cavitation inside the intestinal crypt and the depth of the crypt are significantly shortened,and lymphocyte infiltration is also shown.