Study On Antioxidative Activity And Enhanced Immune Activity Of The Enzymatic Hydrolysate Of Sea Cucumber Gonad

The North sea cucumber farming in the south in Fujian has been fully developed,and the yield increased year by year,has become one of the main varieties of Fujian marine culture,but the sea cucumber processing way is single,resources not used effectively.Sea cucumber is a sea cucumber in the reproductive period of mature gonad organs,containing sea cucumber eggs and sperm,known as the sea cucumber in the gold.The sea cucumber gonads which contains rich nutrients and various bioactive substances,these bioactive substances have antioxidant,enhanced immunity,relieve blood pressure,reduce blood fat and other biological functions.In this study,the sea cucumber gonads as raw material,using exogenous protease hydrolysis,antioxidant activity of sea cucumber by gonadal enzyme detection in mice hydrolysate and enhance immune activity,provides the reference for the industrialization development of sea cucumber gonad products and deep processing.The main research contents and conclusions are as follows:1.The effect of solution through the comparison of various enzymes,determined using enzyme of papaya protease.Using single factor experiment and response surface optimization,the hydrolysis conditions were determined as 14 h,3000 U/g,and the feed liquid ratio of 1:40,p H value was 7,and the enzymatic hydrolysis temperature was 55.The enzymatic hydrolysate of the sea cucumber gonads was obtained after centrifugation,concentration and drying,The sea cucumber gonad hydrolysates of main components were protein 48.4%,ash 16.8%,crude fat 12.4%,water 9.8%,total sugar 8.4%,holothurin 1.1%.2.The activity of free radical scavenging activity of the enzymatic hydrolysate of sea cucumber in vitro was related to the concentration of free radical.The IC₅₀ value of·OH is 5.92mg/m L,and the regression equation was y=0.039x+0.269,R²=0.994,The IC₅₀ value of DPPH·is 3.42 mg/m L,and the regression equation is y=13.9x+7.077,R²=0.999.3.Study of antioxidant activity in vivo of mice with ethanol oxidative damage model.The experimental results show that the sea cucumber gonad hydrolysates on the growth of mouse weight had no obvious effect;It can significantly reduce the content of MDA in sera of mice(P<0.05)and significantly decreased the content of MDA in liver of mice(P<0.01);It can significantly reduce the content of protein carbonyl in the liver of mice(P<0.01);It can significantly enhance the serum SOD activity(P<0.05)and a significant increase in mouse liver SOD activity(P<0.01);It can significantly improve the content of mouse liver GSH(P<0.05).The sea cucumber gonad hydrolysates showed good antioxidant activity in vivo antioxidant experiments,has antioxidant function.4.The sea cucumber gonad hydrolysates immune experiment results show that the weight of mice and the organs of sea cucumber gonads enzyme(liver,spleen and thymus)was not affected by delayed type,hypersensitivity test of sea cucumber gonad hydrolysates can significantly improve the paw thickness of mice(P<0.05);the serum hemolysin test of the sea cucumber gonad hydrolysates can significantly increase the number of antibody in mice(P<0.01);mice by carbon clearance experiment of sea cucumber gonad hydrolysates could promote the phagocytic index of mice abdominal macrophage(P<0.05).The research shows that the product of the gonad of sea cucumber has the function of regulating the immune function.