Development And Research Of Health Food Yizhi Chewable Tablets

Objective:Yizhi chewable tablets is a kind of health food with the functions of filling marrow,nourishing blood and tonifying deficiency,tranquilizing and replenishing intelligence,which is based on Liuwei Dihuang pills.It provides experimental basis for the further development of Yizhi chewable tablets.In this study,the prescription compatibility,preparation technology,functional evaluation,safety evaluation and quality standard of Yizhi chewable tablets were systematically studied.Method : Through literature research,the components of each drug and the mechanism of related drugs were analyzed to test the rationality of the thinking of the prescription;meanwhile,functional experiments were used to optimize the prescription dosage and orthogonal experiment was used to optimize the technology;scopolamine model mice were tested by platform jumping experiment,dark avoiding experiment and Morris water maze experiment Prescription improves memory function.In addition,the potential target of Yizhi chewing tablets to improve memory impairment was predicted and screened by using the network pharmacology research technology and molecular docking technology,which provided theoretical basis for its potential mechanism.On this basis,the combination characteristics of prescription of Yizhi chewable tablets were further explained by using molecular docking technology to verify the reliability of target prediction.After the pretreatment of the raw materials in the prescription,orthogonal design test was adopted in the extraction process.The content of total saponins was investigated.The soaking time,the time of decoction,the amount of water added and the number of times of decoction were four factors.Through the design of orthogonal test,the process parameters of water extraction were determined,and the best water extraction process was selected and verified.In the forming process,water extract is the main raw material.When choosing the appropriate auxiliary materials,the filler,adhesive and flavor straightener are optimized and selected in the amount of use by single factor test method.The optimal scheme is determined.The preparation method is simple,sweet and delicious,and the edible and easy to carry.The process validation is carried out according to the optimal process.After 30 days of continuous gavage of ICR mice,the mice in other groups except the blank group were given 5 mg/kg scopolamine 5 mg/kg by intraperitoneal injection,and each group was injected with scopolamine 5 mg/kg,so as to prepare the memory impairment model.After modeling,the changes of learning and memory ability of mice in each group were further evaluated by jumping off the platform experiment,dark avoiding experiment and Morris water maze experiment.The changes of ACh,ache and ache in brain tissue of mice were further evaluated The changes of the content of(Ch AT)were measured to evaluate the function of Yizhi chewable tablets in improving memory in mice.The safety evaluation of Yizhi chewing tablets was studied.First,when the mice and SD rats were given,the maximum dose(46g of raw medicine /kg)and the maximum gastric perfusion(20 ml/kg)were used BW)were given gastric administration,and the poisoning reaction of animals was recorded for 14 days.Then,the rats were given three doses of 2G raw medicine / kg,4G raw medicine /kg and 8g raw medicine /kg for 30 days.During the period,the general situation,body weight and food intake of the animals were dynamically observed.After the test,the rats were dissected,hematology,blood biochemistry,organ index and tissue index Pathological examination.In the quality control study,the sensory evaluation was used to evaluate the visual,olfactory and taste of the product.In this study,the UV spectrophotometry was used to determine the total saponins in Yizhi chewing tablets;the HPLC method was used to establish the content determination method of polyglycoside in yizhichewable tablets;the index components of Polygala were determined by HPLC The quality standard of Yizhi chewing tablets was established by the determination of content.Result : The Yizhi chewing tablet has the functions of filling the lean pulp,nourishing blood and toning deficiency,and soothing the mind and promoting the intelligence.The network pharmacology research shows that the main active components of Yizhi chewable tablets are through the interaction pathway of active ligand receptor,vascular endothelial growth factor signal pathway and cholinergic synaptic signal pathway.The Yizhi chewing tablets may be regulated by proliferation and apoptosis,inflammatory response and inflammatory response through the active component cells such as cousterol,?-glutasterol,diosgenin,and haifengtengone AKT1,VEGFA,PTGS2,ACh,ache,and chat target play an important role in improving memory.The best preparation technology of Yizhi chewing tablets is: soak the raw materials in water for 1 hour,add to 10 times the volume of water,cook twice,boil for60 min each time,filter them,concentrate into extract and get the extract powder by decompression drying.After 100 sieves,add 17% lactose,6% microcrystalline cellulose and 17% xylitol in the prescription,and use 95% ethanol as the mixture As wetting agent,the wet granulation was passed 20 mesh sieve,the obtained particles were vacuum dried at 50 ? until the moisture was about 5-8% and 14 mesh sieve.Finally,the prepared particles were added to the tablet by mixing 6% silica and 2%magnesium stearate to press the tablets,that is,the weight of the film was controlled at 910 ± 3% Mg,the hardness of the film was 5-7kg.n,and the weight difference was checked to be qualified.The plain film coated premix(white and green)was used to feed the plain film into the tablet The clothes are bright green and beautiful.The results of functional evaluation showed that Yizhi chewing tablet can significantly prolong the latency of scopolamine mice 'platform jumping,reduce the number of errors within 3 minutes,significantly prolong the incubation period of model mice,and significantly reduce 5 The number of errors in min can shorten the escape latency,increase the number of platform entry and decrease the escape latency in the positioning navigation of mice.It is suggested that the Yizhi chewing tablet can significantly improve the learning and memory disorders caused by scopolamine.The content of ache and chat in the brain tissue of scopolamine mice was significantly reduced in the group of 4G raw medicine / kg and 8g raw medicine / kg,and the content of ache and chat in the brain tissue of scopolamine mice was significantly increased in the 8g group.The Yizhi chewing tablet could significantly improve the learning and memory ability of scopolamine mice model,which may be through regulating cholinergic pathway,increasing ACh,chat and reducing ache content The learning and memory impairment of mice induced by scopolamine was improved.In terms of safety evaluation,the tablets have no obvious toxicity and side effects.There is no obvious damage to many important organs such as new,liver,lung,kidney and spleen.The maximum safety dose is 46 g of raw medicine / kg,which is150 times of the clinical human dose.It can be seen that the tablets are safe and have the popularization.In terms of quality standard,the total saponin content of Yizhi chewing tablets was determined by UV spectrophotometry.The content of saponin in the leaves of Yizhi chewing tablets was established by HPLC.The quality standard draft of Yizhi chewing tablets was formulated according to the national standard(gb16740-2014).Conclusion : The Yizhi chewable tablet developed in this paper is a health product with the main effect of improving memory impairment.Through a large number of data mining,network pharmacology and functional evaluation methods,the rationality of prescription compatibility of Yizhi chewable tablets was comprehensively elaborated,the process parameters were determined,the function and safety of Yizhi chewable tablets in improving memory impairment were evaluated,the quality standards were formulated,and the development of Yizhi chewable tablets was generally completed,which is worthy of further promotion and application.