Effect Of Allicin On REDOX Of Tumor Cells

In recent years,epidemiological investigation and experimental studies have shown that allicin has anti-inflammatory,antibacterial,anti-tumor and other physiological functions,and most of these functions are related to the activity of allicin regulating the body's redox level.It has not been reported to explain the redox level in tumor cells and the spatiotemporal expression of APE1 in tumor cells.Therefore,the effects of characteristic nutrients in garlic on the imbalance of redox level of tumor cells were studied.The content and distribution of ROS(reactive oxygen species)and APE1 in tumor cells were deeply studied.The effect and mechanism of allicin on redox of typical tumor cells were explored,which provided a theoretical basis for the efficacy of characteristic nutrients in garlic.In this paper,we established a PMA(Phorbol 12-myristate 13-acetate)oxidative stress model,constructed a two factor central energy response surface model with ROS as a single evaluation index,and speculated the optimal incubation conditions.It was concluded that 10 mg / L allicin could protect cells from the increase of ROS content caused by PMA oxidative stress when incubated 24 h in advance.The expression of GSH-Px(glutathione peroxidase)and other five redox biomarker enzyme systems in different allicin incubation groups were determined,and the weight factors were calculated to form a comprehensive evaluation system,so as to solve the complex problem of antioxidant capacity evaluation in biological system.In the experimental group,after incubation with 10 mg / L Allicin for24 h in advance,the cell group interfered by PMA got the highest score,which was consistent with the result simulated by response surface model.The expression levels of MDA and 8-oxo-d G in He La cells were determined.It was speculated that allicin could protect He La cells from lipid peroxidation and DNA peroxidation after incubation with 10 mg / L Allicin for 24 h.The results of these two adverse effects were inversely correlated with the comprehensive score.It is speculated that allicin regulates the redox level of the body,which may be related to the repair pathway of gene damage in the body.This provides a scientific direction for the follow-up research on the mechanism of allicin protecting cells from PMA oxidative damage.In order to visualize the effect of allicin on the expression of ROS and APE1 enzymes(regulating gene damage and redox level)in tumor cells,we developed a DNA frame probe based on DNA molecular simulation data,which can simultaneously detect multiple substances in tumor cells.The constructed DNA tetrahedral framework is considered to be a multi-functional 3D structure,which can be assembled by PCR gradient annealing.The structure has structural controllability,cell compatibility,high stability,low toxicity,and can specifically present the spatiotemporal co localization of ROS,APE1 enzyme and other substances in cells in the form of fluorescence intensity.The results show that the time-space changes of various substances and the relationship between them and mitochondria can be visualized by DNA nanoframe.It is concluded that the expression of ROS and APE1 enzyme has a synergistic relationship not only in dose,but also in space.At the same time,we verified the above conclusion that allicin can regulate ROS content and APE1 enzyme expression in tumor cells.What's more innovative is the concept of Co-location of ROS and APE1 in space and time.Therefore,we speculate that allicin can affect APE1 enzyme expression by regulating ROS content in tumor cells,and then change the gene repair ability of tumor cells,Finally,the protective mechanism of redox and gene oxidative damage in cells is formed.