Study On The Determination Of Coenzyme Q₁₀ By Adsorption Voltammetry

ObjectiveTo study the electrochemical behavior of coenzyme Q10 on glassy carbon electrode by adsorption voltammetry,and based on this,to establish a simple and convenient new analysis method of coenzyme Q10.MethodsThe adsorption behavior of coenzyme Q10 on glassy carbon electrode was investigated by cyclic voltammetry,and the electrochemical process of coenzyme Q10 was characterized by cyclic voltammetry,Choosing reaction temperature coenzyme Q10 dissolve the ultrasonic time ratio of electrolyte solution(methanol,ethanol)as a condition of experimental optimization,and the use of response surface software Box-Behnken design scheme on the basis of single factor analysis to analyze the optimum reaction conditions of coenzyme Q10 standard solution using ethanol directly dissolved method configuration,in the work of glassy carbon electrode was three electrode system adsorption voltammetry measuring the concentration of coenzyme Q10,with reference to the peak height of cyclic voltammetry scanning of coenzyme Q10 content standards Through the current response value,the linear equation of the relationship between the reaction of different concentrations of coenzyme Q10and the current value of adsorption voltammetry was fitted,so as to make a quantitative analysis of the concentration of coenzyme Q10,and then the differential pulse voltammetry was used to reduce the detection limit of the detection of coenzyme Q10 and improve the detection sensitivity.ResultsThe electrochemical behavior of coenzyme Q10 on the glassy carbon electrode was controlled by adsorption.The peak current was linearly related to the scanning rate,and the linear equation was y=2.16+131.08x(r²=0.997).Comply with the relevant adsorption-controlled electrochemical processes.The preenrichment time of coenzyme Q10 tended to be stable at 25 min.The adsorption process of coenzyme Q10 on the surface of glassy carbon electrode belongs to the adsorption process of reactants and is a weak chemisorption process.Electrode reaction transferred electron number n=2,in the process of adsorption on the surface of the electrode?=3.07*10^-11mol/cm²,should be unsaturated adsorption.The results of single factor analysis were as follows:ultrasonic time of the sample was 2 min,experimental temperature was 25,and solvent ratio was 1:1.The optimal conditions optimized by response surface methodology were as follows:ultrasonic time 1.88min,temperature 23.3,solvent ratio 1.1:1.The regression results of cyclic voltammetry current response to coenzyme Q10 concentration were as follows:I=1.583+2.723lg C(r²=0.996),the linear range was 25 nmol/L to 25?mol/L,LOD=19 nmol/L.The regression results of the current response of the differential pulsed voltammetry method to the concentration of coenzyme Q10 were as follows:I=0.389+0.0016C(r²=996),with a linear range of 5 nmol/L to 250 nmol/L,LOD=3.27 nmol/L.ConclusionsThe electrochemical mass transfer behavior of coenzyme Q10 on glassy carbon electrode surface is controlled by adsorption from many angles.The current response value of coenzyme Q10 was significantly improved by using the single factor analysis optimization method and response surface optimization method.Coenzyme Q10 was quantitatively analyzed by adsorption voltammetry,and it was found that the concentration of Coenzyme Q10 and its corresponding current value showed good linear correlation and stability in a certain concentration range.Compared with traditional methods,this method has certain advantages in terms of simplicity and economy.