| Chemiluminescence(CL)is a type of molecular luminescence spectroscopy.Its principle is mainly based on that the concentration of the analyte in the chemical detection system and the CL intensity of the system are linear and quantitative under certain conditions.Because of high sensitivity and high analysis efficiency,it is widely used in the determination of a variety of samples.Luminol,as the most classic CL reagent,has been widely used in biological analysis,but it is restricted due to their inability to combine with most substances.In addition,compared with luminol,N-(4-aminobutyl)-N-ethylisoluminol(ABEI)has higher activity,which makes ABEI more conducive to the reaction with other reagents.Due to these advantages,some sensors based on ABEI have been gradually developed in recent years.Amino acid is one type of biologically active molecules that construct biological organisms.It is the basic material for building cells and repairing tissues.Among them,tyrosine(Tyr)is one of the most important component of protein in the human body and plays a very important role in the growth and development of the human body.For example,it helps regulate appetite,pain sensitivity and the body's response to stress.Therefore,the establishment of a simple,rapid and efficient test method of Tyr in food has many practical significances.Cysteine(Cys)is also a non-essential amino acid in the human body.Due to its structure containing sulfur,it has a good antioxidant effect and is widely used in clinical medicine and biochemical research fields.Based on the important role of two non-essential amino acids in the human body,this article will use ABEICL system to detect Tyr and Cys.In addition,the detection of Tyr in dairy products and the detection of Cys in water products and dairy products have been realized.This paper is divided into three chapters:The first chapter gives a corresponding overview of Tyr and Cys,mainly introduces its detection significance and the current research status of its detection by CL method,and objectively evaluates and compares various detection methods.The second chapter systematically studied a new detection method of Tyr based on the principle of CL resonance energy transfer of sodium fluorescein and ABEI,the experimental conditions have been optimized,and the selectivity and anti-interference ability of this method for Tyr have been discussed.Finally,under the best experimental conditions,the detection of Tyr in dairy products was successfully achieved.The linear concentration range was 3.0×10-8mol/L to 3.0×10-5mol/L,and the detection limit was as low as 2.4×10-8mol/L..Chapter 3 discusses a new type of polytetrabromophenylporphyrin(Phbp)that can promote dissolve oxygen in water was synthesized,and it is applied to ABEI-sodium fluorescein system to enhance the intensity of CL.The mechanism results show that Phbp can increase the conversion of dissolved oxygen into active oxygen free radicals in water.Therefore,this CL system does not require hydrogen peroxide.Based on this principle,an ultra-sensitive,low-cost,simple and practical Cys method was developed.Under the best experimental conditions,Cys showed a good linear relationship in the range of 0.1-10?mol/L.This method can be further applied to the detection of Cys in aquatic products and dairy products,and good experimental results have been obtained. |
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