Synthesis And Chemiluminescence Study Of N-(Aminobutyl)-N-(Ethylisoluminol)and Hemin Dual-Functionalized Graphene Hybrids

In this dissertation, the state of arts in the field of chemiluminescence (CL), functionalization of graphene and chemiluminescence functionalized nanomaterials(CF-NMs), as well as its applications in bioanalysis were reviewed. In recent years, many nanomaterials such as metal nanoparticle, SiO2nanoparticle, carbon nanotube and graphene have been used to synthesize CF-NMs according to unique characteristics of nanomaterials including chemical reactivity, catalytic property, biocompatibility and ease of self-assembly. Most of the reported CF-NMs have made great progress in improving sensitivity and reducing analysis time. However, in order to achieve higher sensitivity and wider linear range, CF-NMs with high CL, good solubility and stability are highly desired. The aim of this dissertation is to explore the synthesis of N-(Aminobutyl)-N-(ethylisoluminol)(ABEI)and hemin dual-functionalized nanomaterials. A novel dual-functionalized graphene hybridwas obtained and its CL property was investigated.The main results are as follows:A facile strategy for the synthesis of chemiluminescence and its catalyst dual functionalized graphene hybrids is reported. ABEI and hemin dual-functionalized graphene hybrids (A-H-GNs) were successfully prepared. The surface chemical components were investigated by employing UV-visible spectroscopy, X-ray photoelectron spectroscopy (XPS), FT-infrared spectroscopy, Raman, and inductively coupled plasma-atomic emission spectrometry (ICP-AES) techniques. The results demonstrated coexistence of ABEI and hemin on the surface of as-synthesized A-H-GNs. The assembly mechanism of graphene with ABEI and hemin was explored by using atomic force spectroscopy (AFM) and Fluorescence spectroscopy (FL). The results reveal that the as-prepared hybrid is multilayer decorated with ABEI and hemin molecules based on non-covalent interactions among graphene, ABEI and hemin withABEI/hemin/ABEI...multilayer stacking structure. Furthermore, CL and ECL behavior of A-H-GNs were studied by static injection method, cyclicvoltammetry,and multi-potential stepstechnique. The results indicated that as-prepared A-H-GNspossess excellent CL and ECL properties. Moreover, as-prepared A-H-GNs also exhibited good stability and high water solubility. This work has discovered a novel approach for preparing CL and its catalyst dual functionalized graphene hybrids. Taking advantage of the carbonyl groups of hemin, the A-H-GNs possesses high water solubility and good stability, which allows further exploration of graphene based CL sensors in the biological field.