Research On The Molecularly Imprinted Electrodes Decorated By Nanomaterials For The Determination Of Four Kinds Of Pesticide

Four kinds of molecularly imprinted polymer membrane electrochemical sensors were prepared using four different sensitizing nanomaterials by two methods in order to achieve reliable,rapid,sensitive and inexpensive detection of amide fungicides(flumorph and benaxyl)and pyrethroids(tetramethrin and cypermethrin).Then,the prepared materials and imprinted sensors were characterized and the practical applicability of imprinted sensors was studied.The details are as follows:Firstly,the glassy carbon electrode decorated by surface-corrosive graphene oxide doped with polyaniline(SCGO@PANI/GCE)was prepared by combining the characteristics of surface-corroded graphene oxide Graphene oxide with good conductivity and good surface corrosion and polyaniline with good conductivity and adhesion.Then the in-situ polymerization method was employed to prepare the flumorph molecularly imprinted polymer film electrochemical sensor based on surface corrosion graphene oxide doped with polyaniline(FM-MIM/SCGO@PANI/GCE)using flumorph as the template molecule and acrylamide(AM)as the functional monomer.The detection range of the sensor is 5.0×10^-9?1.0×10^-6mol/L and 1.0×10^-6?1.0×10^-4mol/L.The detection limit of the sensor is 9.2×10^-10mol/L(S/N=3).In the FM test of vegetables,the recovery rate of standard addition is between 98.16%?103.90%and the RSD is not more than 3.72%.2?Firstly,the surface of the glassy carbon electrode was decorated by graphene with good conductivity doped with gold tungsten heteropoly acid with excellent electrocatalytic activity(G@Au W-HPA/GCE),then the in-situ polymerization method was used to prepare the benaxyl molecularly imprinted polymer film electrochemical sensor based on graphene doped with gold tungsten heteropoly acid(BA-MIM/G@Au W-HPA/GCE)using benaxyl(BA)as the template molecule for the detection of benaxyl in vegetables.The detection range of the sensor is5.0×10^-10?5.0×10^-8mol/L and 5.0×10^-8?3.0×10^-5mol/L.The detection limit of the sensor is 2.6×10^-10mol/L(S/N=3).The recovery rate of standard addition in big cabbage and small cabbage is between 98.90 and 101.3%and the RSD is less than or equal to3.87%.3?Compared with the in-situ polymerization method,the electropolymerization method can shorten the experimental period by half because it is more efficient.The tetramethrin molecularly imprinted polymer membrane electrochemical sensor(TET-MIM/Rh/GCE)based on the dendritic rhodium modified was prepared using electropolymerization method with dendritic rhodium as sensitizing material?tetramethrin as the template molecule and o-aminophenol as the functional monomer.The detection range of the sensor is 5.0×10^-10?5.0×10^-8mol/L and 5.0×10^-8?2.0×10^-5mol/L.The detection limit of the sensor is 2.6×10^-10mol/L(S/N=3).The recovery rate of standard addition used for TET detection in environmental water samples is between98.50?106.20%and the RSD is less than or equal to 4.15%.4?In order to obtain a cypermethrin molecularly imprinted electrochemical sensor with stable?efficiency and environmentally friendly.Firstly,a screen-printed carbon electrode(PG/SPCE)modified with porous graphene as sensitizing material was selected,then cypermethrin molecularly imprinted polymer membrane electrochemical sensor based on porous graphene modification modified was prepared using the in-situ polymerization method with cypermethrin as the template molecule and2-p-vinylphenyl-pyridine and?-methacrylic acid as the double functional monomer.The detection range of the sensor is 3.0×10^-9?1.0×10^-6mol/L.The detection limit of the sensor is 2.1×10^-9mol/L(S/N=3).The recovery rate of standard addition used for the detection of CYP in Cuihu water samples is between 99.30?105.67%and the RSD is less than or equal to 5.55%.