| Antibiotics are an important chemical reagent.It was once considered a major achievement in microbiology and biology.Antibiotics are mainly used in medical treatment,biological research,agriculture,animal husbandry and food industry which have promoted the development of human beings.However,the problems casued by the abuse of antibitics have seriously threatened people's health and environment.Therefore,it is of great scientific value and pratical significance to realize the accurate detection of antibitics.Because the electrochemical sensor have the feature ofhigh sensitivity,good selectivity and simple operation,they is widely used in various fields.The graphene has good electrical conductivity.The special structure also allows graphene to support other substances.The single-walled carbon nanohorns?SWCNHs?is also a unique carbon nanomaterials.Its application in the transport of intracellular deugs,proteins and DNA molecules has unparalleled advantages over oridinary nanomaterials.And the SWCNHs has good electrical conductivity and chemiacl stability.In this thesis,based on the excellent properties of these two carbon nanomaterials,several new electrochemical sensors are assembled by combining them with electrochemical technology.The details of these research are as followed:1.Prepapration of RGO-PEI used graphene and PEI as raw materials.And the RGO-PEI was used to modify the surface of glassy carbon electrode because of its excellent characteristics.The oxytetracycline molecular imprinting sensor and the chlorotetracycline molecular imprinting sensor by molecular imprinting method are constructed.And the accurate and rapid detection for oxytetracycline and chlorotetracycline was carried out.These two kinds of electrochemical sensors were characterized by the cvclic voltammetry?CV?and liner sweepvoltammetry?LSV?.The wide linear ranges of 1.0×10-6mol/L1.0×10-4mol/L for oxytetracycline were obtained,with corresponding detection limits of 3.33×10-7mol/L.Finally,the electrochemical sensor was successfully applied in the practical samples to detect oxytetracycline,with the recovery of100.4%102.6%,RSD 4.44%.The sensor showed high accuracy.It has good linear relationship amongst 5.0×10-7mol/L1.0×10-4mol/L,with detection limits of 1.67×10-7mol/L.The electrochemical sensor was successfully applied in the practical samples to detect oxytetracycline,with the recovery of 102.7%104.7%,RSD 2.94%.The sensor also showed high accuracy.2.The functional SWCNHs are used to modify the surface of glassy carbon electrode and we structured SWCNHs/GCE.The functional SWCNHs have electro-catalytic activity to the reduction of chloramphenicol.it can specialy distinguish chloramphenicol.The linear detection range of chlorotetracycline is 1×10-7mol/L1×10-4mol/L,with corresponding detection limits of 3.33×10-8mol/L?S/N=3?.The electrochemical sensor is successfully applied in the practical samples to detect chlorotetracycline,with the recovery of 102.7%106.8%,RSD 3.23%.The sensor showed high accuracy. |
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