Study On Preparation And Anti-tumor Application Of Nano-micelle With Adjustable Morphology

In tumor-targeted therapy,the shape design of nanocarriers has gained growing interests.Generally,rod-shaped nanocarriers are more efficient than spherical nanocarriers in vascular transport(from blood vessels to interstitial)and interstitial transport(from interstitial to target cells).transvascular transport—movement from vessels to the interstitium—and interstitial transport—movement through the interstitium to target cells.That is,rod-shaped nanomicelles are more likely to leak from tumor blood vessels and have stronger tumor penetration.At the same time,larger-sized spherical nanocarriers have longer residence time at the tumor site than rod-shaped nanocarriers.To this end,we have developed a strategy that can change the shape of nanocarriers "according to local conditions",so that nanocarriers can exhibit a rod-like morphology with a high aspect ratio in blood vessels and tumor interstitium,and a spherical morphology in the depth of the tumor.It is worth noting that through a combination of "bottom-up" and "top-down" preparation of nanoparticles,a series of rod-shaped micelles with different length-to-diameter ratios were prepared ingeniously and concisely.This "temporary" rod-shaped micelle responds to the tumor microenvironment temperature by chemical crosslinking of polycaprolactone(PCL)segments,thereby triggering the conversion of rodshaped micelles into spherical micelles.In addition,based on our experimental results,we constructed a phase diagram of the relationship between fiber stretching and nano-micelle aspect ratio to ensure the reproducibility of the experimental results,and at the same time help us better understand the fiber stretching and nano The relationship between the aspect ratio of micelles.Evaluation at the cellular level shows that the rod-shaped micelles have good cell compatibility.Evaluation at the animal level shows that the rod-shaped micelles exhibit good biocompatibility and inhibit tumor growth in vivo by x% when intravenously injected at ordinary doses.All in all,we design a simple and effective method for obtaining rod-shaped nanomicelles,and then provide a strategy for nanomicelles to be easy to leak,strong to penetrate,multi-retain and with the potential to improve therapeutic outcome.