Preparation And Study Of Colonic Targetedslow-release Nanofiber Membranes By Coaxial Electrospinning

Currently,electrospinning is one of the most important methods to fabricate microfiber.The prepared microfiber has the characteristics of small fiber fineness,large specific surface area and high porosity,etc.Site-specific drug release and slow-release system have become the focus of medical oral medicine research,which can effectively alleviate many problems of traditional drug release,such as great side effects and poor therapeutic effect.In this paper,the drug-loading nanofiber membrane was prepared by electrospinning technique,which possess colon-targeted sustained-release function.Meanwhile,exploring the localized controlled release properties,and a series of cell activity experiments were carried out.Firstly,the E-S100/E-RS100 nanofiber membrane loaded with sinomine hydrochloride(SH)with p H-sensitive Eudragit S100 as shell materials and Eudragit RS100 as core materials was prepared by coaxial electrospinning technology.The surface morphology,internal structure and chemical structure of the fibers were characterized by scanning electron microscopy(SEM),transmission electron microscopy(TEM),infrared spectroscopy(FTIR)and X-ray diffraction(XRD).The prepared drug-loaded nanofibers have obvious shell/core structure,smooth surface and uniform diameter distribution,and the drug is completely wrapped in the nanofibers.The results of in vitro drug release showed that the release rate of SH was less than 20% in gastric acid simulation solution,and it was transferred to colon simulation solution for a sustained release time reached 240 min,and the release rate was more than 90%.The release dynamics simulation is consistent with first-order release.Blood compatibility and cytotoxicity tests showed that the nanofiber membrane had no hemolytic effect,good cytocompatibility and the material has no cytotoxicity.Secondly,the E-S100/PCL nanofiber membrane loading camptothecin(CPT)was prepared with Eudragit S100 as shell materials and hydrophobic polymer PCL as core materials.The surface morphology and chemical structure of the fibers was characterized by scanning electron microscopy(SEM),infrared spectroscopy(FTIR),X-ray diffraction(XRD).The prepared drug-loaded nanofibers have smooth surface,no bead adhesion and uniform diameter distribution,and the drug is completely wrapped in the nanofibers.The results of in vitro drug release showed that the release rate of CPT was about 10% in gastric acid simulation solution,and it was transferred to colon simulation solution for a sustained release time reached120 h,and the release rate was more than 90%.The release dynamics simulation is consistent with Higuchi release model.Blood compatibility and cytotoxicity tests showed that the nanofiber membrane had no hemolytic effect,good cytocompatibility and the material has no cytotoxicity.The experiments on anticancer cells showed that the drug-loaded nanofiber membrane had excellent ability of anticancer cells.Finally,the E-S100/E-RS100&PCL nanofiber membrane loading Luteolin(LUT)was prepared with Eudragit S100 as shell materialsand E-RS100 and PCL as core materials.The surface morphology and chemical structure of the fibers were characterized by scanning electron microscopy(SEM),infrared spectroscopy(FTIR),X-ray diffraction(XRD).The prepared drug-loaded nanofibers have smooth surface,no bead adhesion and uniform diameter distribution,and the drug is completely wrapped in the nanofibers.The results of in vitro drug release showed that the release rate of Lut was less than 6% in gastric acid simulation solution,and it was transferred to colon simulation solution for a sustained release time reached40 h,and the release rate had a top speed of 70%.The release dynamics simulation is consistent with Higuchi release model.Blood compatibility and cytotoxicity tests showed that the nanofiber membrane had no hemolytic effect,good cytocompatibility and the material has no cytotoxicity.