Electrospinning Technology To Prepare Fiber Membrance For Skin Repair

As the largest organ to prevent pathogens from invading the body,the skin will cause trauma due to mechanical and chemical damage.With the in-depth study of the skin wound healing mechanism,there is more demand for the function of wound dressings.Electrospinning is a simple technology for preparing nanofibers.The prepared fiber membrane has a highly interconnected porous structure and a partial extracellular matrix structure,which can meet the various needs of skin wound repair.Nanofibers prepared by coaxial electrospinning provide a broad prospect for the study of drug release control due to their unique core-shell structure.This paper uses polycaprolactone(PCL),polyethylene oxide(PEO)and polyvinyl alcohol(PVA)with good biocompatibility as spinning aids,and the biologically active Spider silk protein(Ss),which has a wide range of the combination of spectral antibacterial property and Periplaneta Americana extract(PAE)that promotes the secretion of growth factors,the PCL/PEO/Ss@PVA/PAE coaxial nanofiber membrane with PAE loaded on the core layer and Ss loaded on the shell layer was prepared,and the fiber membrane was explored.Physical properties such as mechanical and hydrophilic properties,in vitro degradation and in vitro drug release,as well as antibacterial effects and cell compatibility.The main research contents are as follows:(1)By adjusting the process parameters,a fiber membrane with a core-shell structure with a fiber diameter of 500-1000 nm was prepared.The process parameters are as follows,the spinning receiving distance is 20 cm,the spinning voltage is 12-16 kV,and the core layer PVA concentration is 10 wt%,The shell layer PCL concentration is 8,9,10 wt%,the PEO concentration is 2,2.5,3 wt%,the maximum feeding rate,the core layer feeding rate is 0.36 mL/min,and the shell layer feeding rate is 1.8 mL/min.(2)Optimize the selected better spinning conditions,and successfully load Ss and PAE on the shell and core layers of the fiber,respectively,to obtain a regenerated Ss nanofiber membrane(PPS)and a drug-loaded Ss nanofiber membrane(PPS@PAE).With the increase of Ss content,the diameter of PPS fiber decreases from 420 nm to 350 nm,and the thickness of the shell decreases from 220 nm to 90 nm.The diameter of PPS@PAE fiber is reduced from 350 nm to 280 nm,and the thickness of the shell layer is reduced from 115 nm to 70 nm.(3)Both nanofiber membranes have good thermal stability.The mechanical and hydrophilic properties of the fiber membrane were improved by adding Ss.When the Ss content is 20 wt%,the swelling rate of the fiber membrane can reach 142%and 150%,and the water vapor transmission rate is 1843 g/m2·24h and 1802 g/m2·24h respectively.(4)The coaxial fiber structure can effectively control the release of PAE.PPS@PAE has good antibacterial properties,and the 48h cytotoxicity of the two nanofiber membranes is above 90%,which has good biocompatibility,and the addition of Ss and PAE can effectively promote cell proliferation.Compared with the unloaded Ss nanofiber membrane,the cell proliferation effects of PPS and PPS@PAE with Ss content of 20wt%increased by 20%and 25%,respectively.