| At present,the main method for treating arthritis and other diseases is artificial hip replacement.3D printing has the advantages of fast molding speed,the ability to manufacture complex parts,and the high precision of the printed structure.Many 3D printing materials add coatings to improve the performance of the materials.Layers,such as bioactive coating hydroxyapatite(HA),but the safety and biocompatibility of the coating still need to be confirmed.This study evaluated the biosafety and effectiveness of 3D printed acetabular cup materials with HA-coating and without HA-coating,and judged the safety and effectiveness of HA-coating on 3D printed titanium alloy materials.Impact.1)Observe and compare the difference between the material with HA-coating and the material without HA-coating by scanning electron microscope.There is a big difference between the two under the scanning electron microscope.The surface of the material without HA-coating is rough but the structure is relatively clear,and the aperture has a certain rule to follow,while the surface of the material with HA-coating is all covered by the fragments of HA,and the aperture is The structure is hindered to a certain extent;the porosity of the two is calculated and compared,the porosity of the material without HA-coating is 66.0%;the porosity of the material with HA-coating is51.9%.The results show that the HA-coating has a certain effect on the porosity of the material.2)Through cell proliferation,adhesion and cell-material interaction tests,compare the cytocompatibility of two materials with HA-coating and without HA-coating,so as to judge the influence of HA-coating on the cytocompatibility of materials.It shows that the uncoated material is more conducive to cell proliferation than the coated material;the HA-coating material is more suitable for cell adhesion,but it is difficult to observe the cells on the coated material under the scanning electron microscope,indicating that the HA-coating It has a certain effect on cell proliferation and adhesion.3)Through the Bhas42 cell line transformation test,cytotoxicity,irritation,sensitization,acute toxicity,back mutation and mouse lymphoma test,compare the safety of two materials with HA-coating and without HA-coating in vitro and in vivo,and then judge The effect of HA-coating on the biological safety of materials.The results showed that no cell transformation foci were found in the start-up test and cancer promotion test in the HA-coating group and the non-HA-coating group.It can be determined that the HA-coating did not cause the material to cause carcinogenesis;In the reverse mutation and mouse lymphoma test,the coated and uncoated materials showed no toxicity,and it was determined that the HA-coating had no effect on the safety of the material.4)In the bone implantation experiment,the micro CT results of the material taken at the 4th week after implantation found that the two materials have bone ingrowth,and the density of bone ingrowth is difficult to see,indicating that both materials are suitable for bone ingrowth;But in the 8th week of implantation,it was found that the bone ingrowth density of the group without HA-coating was significantly higher than that of the group with HA-coating,which proved that the material without HA-coating has better osseointegration ability.Although HA-coating does not adversely affect the safety of titanium alloy materials,in terms of cell compatibility in vitro,HA-coating has a certain impact on cell proliferation.Therefore,3D printing materials,especially those used in orthopedics,should not only blindly pursue the benefits of coatings,but also consider the adverse effects of HA-coatings. |
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