Synthesis Of Acrylate Copolymer Nanospheres And Their Antibacterial Performance

The spread and infection of bacteria and viruses were seriously threatening the life and health of human being.The development and application of antibacterial materials is to prevent the spread of bacterial pathogens.The research of new and efficient antibacterial materials has rapidly become an important part of the global strategy to alleviate bacterial pathogens infection.Functional polymers play a vital role in the preparation of antibacterial materials.Among them,novel antibacterial polymer materials have attracted more attention due to their broad-spectrum and high-efficiency antibacterial activities.In this thesis,using cheap acrylates as comonomers,the monomer containg cationic quaternary ammonium salts as antibacterial units,and H₂O as solvent,a series of novel cationic acrylate copolymer microspheres with antibacterial activity were designed and synthesized.In addition,by introducing other inorganic antibacterial active centers,their activity was enhanced with synergistic antibacterial effects of double active centers.And they were applied to different antibacterial fields.The main research contents and results are as follows:Firstly.Based on introducing the basic knowledge of mesobacteria,antibacterial agents and their application fields were summarized.Then,the synthesis methods and application fields of some typical polymer antibacterial materials were introduced.Secondly.Blackberry-shape cationic copolymers(BsCCP)nanoparticles were successfully synthesized by one-pot soap-free emulsion polymerization using methyl methacrylate(MMA),N-vinyl-2-pyrrolidone(NVP),[?-(methacryloyl-amino)propyl]trimethyl ammonium chloride(MAPTMA)and?-methacryloyloxy-propyltrimethoxysilane(MAPTMS)as comonomers,H₂O as the solvent,and potassium persulfate(KPS)as the initiator.In addition,the effects of surfactant,initiator,temperature and monomer ratio on the synthesis of BsCCP were studied.Besides,the structure,composition and properties of BsCCP was characterized and analyzed by FT-IR,SEM,XPS,particle size.The antibacterial activity,antibacterial mechanism and cytotoxicity of BsCCP against E.coli and S.aureus were evaluated.The results showed that BsCCP showed good antibacterial activity within 24 h,and had certain concentration dependence.The minimum inhibitory concentration(MIC)of BsCCP against E.coli was 200.00?g/m L,and the MIC against S.aureus was400.00?g/m L.At the same time,BsCCP showed low cytotoxicity.BsCCP was further applied to interior wall coatings to obtain BsCCP-based coatings(BsCCP-C)with antibacterial properties,which also showed excellent antibacterial activity within24 h.Thirdly.Cationic acrylate copovidone-iodine(CACPVI)nanoparticles with double antibacterial activity centers were successfully synthesized by iodine complexation reaction using pyrrolidone(CACPV)as the pyrrolidone(copovidone)unit.The structure,composition and properties were characterized and analyzed by FT-IR,UV-vis,SEM,XPS,particle size and contact angle tests.The antibacterial activity of CACPVI against E.coli and S.aureus was evaluated,and the antibacterial mechanism and cytotoxicity of CACPVI were studied.The results showed that CACPVI with synergistic antibacterial activity showed excellent antibacterial activity within 11 d,and had certain concentration dependence.The MIC of CACPVI against E.coli was 20.00?g/m L,and the MIC against S.aureus was 40.00?g/m L.CACPVI also showed low cytotoxicity.The antibacterial application of CACPVI was further investigated.It was found that the addition of CACPVI to fine chemicals such as ink,dyes and coatings could give it obvious antibacterial properties.Fourth.Raspberry-like cationic acrylate copolymer particles(RsCACPs)were successfully synthesized by one-pot soap-free emulsion polymerization using methyl methacrylate(MMA),glycidyl methacrylate(GMA),styrene(St)and[?-(methacryloyl-amino)propyl]trimethyl ammonium chloride(MAPTMA)as comonomers,water solution as the solvent,and potassium persulfate(KPS)as the initiator.The effects of surfactant,initiator,temperature,MAPTMA content and other factors on the polymer emulsion were investigated.The structure,composition and properties of RsCACPs were characterized and analyzed by FT-IR,SEM,XPS,particle size and contact angle.The antibacterial activities of RsCACPs against E.coli and S.aureus were evaluated,and their antibacterial mechanism and cytotoxicity were discussed.The results showed that RsCACPs showed excellent antibacterial activity within 96 h,and had certain concentration dependence.The MIC of RsCACPs for E.coli was 200.00 mg/m L,and for S.aureus was 400.00 mg/m L.At the same time,RsCACPs showed low cytotoxicity.Further application in the preparation of antibacterial cotton fabric found that after the RsCACPs were coated on the surface of the fabric,the cotton fabric(RPs@CF)had persistent antibacterial activity,and the RPs@CF had good bacterial adhesion resistance.After 50 cycles of washing,the RPs@CF also had certain elution resistance.Fifthly.Selecting the Schiff-base metal complexes(HOSalphen M)as active centers,a novel acrylate copolymer with bounding Schiff-base metal complexes(HSpM@PMNMM)was successfully synthesized by in-situ polymerization using methyl methacrylate(MMA),N-vinyl-2-pyrrolidone(NVP),[?-(methacryloyl-amino)propyl]trimethyl ammonium chloride(MAPTMA)in water,and potassium persulfate(KPS)as the initiator.The structure,composition and properties were characterized by FT-IR,SEM,EDS,XPS,particle size and potential test.The antibacterial activity of HSpM@PMNMM against E.coli and S.aureus was evaluated.The obtained HSpM@PMNMM presented excellent antibacterial activity within 96 h with synergistic antibacterial activity.The antibacterial activity of HSpM@PMNMM against V.mali,the typical apple saprophytic fungi,was also tested.The results of the inhibition zone experiment showed that it had good activity against apple tree rot fungi.In summary,four types of acrylate copolymer microspheres with antibacterial activity were prepared by using quaternary ammonium salt as antibacterial unit and cheap acrylate as comonomer In addition,three kinds of inorganic active centers were introduced into acrylate copolymer microspheres for enhancing their antibacterial activity.The obtained low-cost polymer antibacterial materials could be applied to coating,fabric,fine chemicals and agriculture.The new strategies and methods were provided for the preparation of efficient and durable antibacterial agents.