Preparation Of Light-Responsive Zinc Oxide Composite Material And Its Applications In The Field Of Antibacterial And Wound Healing

Composite materials are the product of interdisciplinary and interpenetration,and become one of the current research hotspots because of their large design freedom,which have been widely used in aerospace,automotive industry,chemical manufacturing and biomedical fields.Herein,in order to effectively sterilize and treat infected wound,two kinds of light-responsive zinc oxide(Zn O)composite materials were prepared.A series of their physicochemical properties and biocompatibility were systematically explored,and finally they were applied to antibacterial and infection wound treatment.All the main research contents are summarized into the following two parts:(1)Inspired by the “lightning war” tactics,a yellow light and electrical stimulation dual-responsive Zn O composite material was created to realize a technical concept of wound antibacterial and tissue repair.We specifically modified Zn O by reducing graphene oxide(r GO)and polydopamine(PDA),and successfully prepared a photoelectric responsive Zn O composite material(PDA-r GO-Zn O,abbreviated as Pr Z)with narrow energy band gap,high photocatalysis,high-efficiency broad-spectrum antibacterial,and preferable biocompatibility.Both in vitro and in vivo antibacterial experiments manifested that the material achieved rapid sterilization after being combined with electrical stimulation.Simultaneously,Pr Z could be excited by yellow light to generate reactive oxygen species(ROS)to further promote the antibacterial efficiency.The applied yellow light was a healthy long-wavelength irradiation,the safety of its photocatalytic antibacterial process thus could be improved fundamentally.Subsequently,animal experiments demonstrated that the system efficaciously regulated the immune response around the wound surface,promoted cell proliferation and the formation of collagen fibers,thereby accelerating infected wound healing.Ultimately,the goal of multiple synergistic antibacterial and accelerated wound healing was achieved.(2)Inspired by traditional moxibustion,a double light(yellow light and near infrared light)responsive carbonized moxa @ zinc oxide(CM@Zn O)was constructed,which combined with drug,light and heat stimulation for the treatment of fungal infection.In this part,the prepared CM@Zn O had a narrow energy band gap(1.55 e V),which could be efficaciously excited by yellow light to produce photocatalysis,thus validly inhibiting the growth of Gram-negative Escherichia coli(E.coli),Gram-positive Staphylococcus aureus(S.aureus)and the typical fungusCandida albicans(C.albicans).Under the action of near infrared light,the good light-to-heat conversion displayed by CM@Zn O further enhanced the effect of inhibiting fungi.In addition,the preferable biocompatibility of CM@Zn O provided a prerequisite for in vivo applications.Finally,through the processing and analysis of bacterial samples from the feet of patients with beriberi,it was shown that the system could effectively inhibit foot fungi growth.