| With the development of human medicine,there has been an in-depth study on the relationship between abnormal concentrations of some indexes in human internal/external environment and human diseases.For the external environment of the human body,the abnormal concentration of hypochlorite in daily water will cause serious damage to the human body.Similarly,nitric oxide in the human environment plays a role in the immune system and vasodilation pathway of the human nervous system.In addition,biological mercaptan glutathione is overexpressed in tumor cells.Therefore,the development of highly sensitive and selective disease marker sensing strategy is of great significance for monitoring human health.In this paper,three sensing strategies for detecting these three disease markers were constructed by using organic synthetic dyes,metal-organic skeleton materials and nanomaterials respectively,and a series of detailed studies are conducted,as summarized below.(1)we developed an efective method to detect hypochlorite acid(HClO)by using methylene blue(MB)derivative(BPY1).BPY1 was selectively oxidized through HClO,and the solution color changed from colorless to blue.In the presence of HClO,the ultraviolet-visible(UV-Vis)spectra and concentration of HClO had a linear relationship with a detection limit of 0.5?mol·L-1.Furthermore,a test paper for HClO monitoring was successfully prepared using the BPY1 probe,and the observed detection limit by the naked eye was estimated at 5?mol·L-1.Additionally,using the BPY1 probe,HClO could also be detected through smartphone colorimetry,and the method showed a good recovery ranging from 98.7 to 104.0%for HClO detection in an actual water sample.Especially for developing countries,such a low-cost and highly sensitive detection method provides a simple and practical method for monitoring HClO in water.(2)Based on o-phenylenediamine(OPD),a sensing strategy for fluorescence colorimetric detection of NO was designed.A method for the preparation of CuCo-based metal-organic framework materials(CuCO-PTC MOF)was proposed.The addition of CuCo-PTC MOF can catalyze the rapid production of hydroxyl radicals by hydrogen peroxide(H2O2)and oxidation of o-phenylenediamine to produce2-diaminophenazine(OPDox)with strong fluorescence emission.The presence of NO will react with OPD to form N-(2-hydrazino phenyl)methylamine,and N-(2-hydrazinophenyl)methylamine will not be oxidized to form OPDox,with fluorescence emission,which further leads to the decrease of fluorescence intensity of the reaction solution.In the presence of NO,there is a linear relationship between the fluorescence emission intensity and the concentration of NO,and the detection limit is0.15?mol·L-1.In addition,the proposed fluorescence colorimetric method can be used to detect NO in biological body fluids,and the practical application potential of the detection strategy is evaluated.(3)Based on metal nanoclusters,gold,silver and copper nanoclusters(GSCN)sensor arrays were constructed for the detection of biological mercaptan in human body.Highly selective recognition and UV-Vis detection of biological mercaptan in biological liquid were realized in the synthesis of GSCN.The addition of GSCN,biological mercaptan synthesized by chemical reduction template method will lead to the combination of metal ions and biological mercaptan in the solution,and then affect the rate of chemical reduction of metals,accompanied by the change of the particle size of metal nanoclusters and the obvious change of UV-Vis absorption of the reaction solution.Through the synthesis of different kinds of metal nanoclusters,four kinds of biological mercaptan were well distinguished by using GSCN sensor array.In addition,the sensor array has the potential to distinguish different concentrations of the same biological mercaptan and mixed biological mercaptan.It is worth noting that the proposed transmitter array can be used to detect biological mercaptan in biological body fluids and verify its practical application potential. |
PDF Full Text Request