Acrylamide Detection Method And Its Application In High-temperature Fried Food

Acrylamide is an unsaturated amide,a colorless and transparent flake crystal,soluble in water,ether,ethanol,insoluble in heptane,benzene,can be used as medicine,pesticides,dyes and coatings,often used in tunnels,oil wells,etc.It can also be used as a soil conditioner for water blocking and sand fixation in large-scale projects.Acrylamide has liver and neurotoxicity.In addition,it has reproductive toxicity to animals,it can damage cell DNA,and can cause rodents to cause cancer.However,there are still no epidemiological experiments and data that can prove that acrylamide can also cause cancer to humans.The International Cancer Agency lists it as a "possible human carcinogen." There is a high level of acrylamide in high-starch foods that have been processed at high temperatures.Therefore,the pollution of acrylamide in foods has been highly valued by governments of various countries.This article takes high-temperature fried food as the research object and uses high performance liquid chromatography to measure it.This paper is divided into three parts:The first part discusses methods to inhibit the formation of acrylamide in food.The second part is to determine the content of acrylamide in fried food.It is necessary to establish a high-performance liquid chromatography analysis method with high accuracy and good convenience.Through the use of this experimental method,it can be better to explore the relevant measures to inhibit the production of acrylamide in food.Experimental results show that controlling the processing temperature within 180°C and adding VC and cellulose can effectively inhibit the production of acrylamide in the food production process,and the effect of adding cellulose is more prominent.The third part of this experiment is to establish a high-performance liquid chromatography method to determine the content of acrylamide in high-temperature processed foods,and to study the monitoring of acrylamide produced after high-temperature processing of foods.By finding suitable liquid chromatography conditions,including changing the mobile phase,using the DAD detector to determine the wavelength,using different methods to activate the elution solid phase extraction cartridge,etc.,establish a rapid detection,high sensitivity,and accurate method.To detect acrylamide in high-temperature processed food and improve its detection recovery rate.By changing the liquid chromatographic conditions,this method can quickly,efficiently and accurately measure the acrylamide content in high-temperature processed foods.The peak shape and separation effect of the obtained acrylamide chromatogram are good.Under these chromatographic conditions,acrylamide has a good linear relationship.This method helps to detect acrylamide in high-temperature processed foods.