Fluorescence Sensing Detection Of Three Hormonal Pollutants Based On Multicolor Upconversion Nanomaterials

Context:Hormone pollutants such as 17?-Estradiol(E₂),Bisphenol A(BPA),and Progesterone(P4)threat to human reproduction,development,and growth.Traditional detection of hormone pollutants is mainly based on large instruments such as liquid phase,liquid mass spectrometry,etc.However,due to the need for professional operation,complex pre-treatment,high refinement degree and high cost,it cannot meet the requirements of rapid detection on site.At present,rapid detection of hormone pollutants has always been a research hotspot,especially in the aspect of multiple detection.At present,immunochromatography,suspension chip multi-channel and other detection technologies have been established,but due to their low sensitivity or the need for large instruments,their further application is limited.With the development of new analytical technology and nanotechnology,nanoparticles with different properties,such as color,size and shape,have been widely used in biological analysis,biological imaging,anti-counterfeiting and other fields.Among them,upconversion material is a new type of nanomaterial which is excited by low energy light and emits high energy light.Due to its advantages such as anti-Stokes emission,narrow characteristic emission peak,optical stability,spectral tunability and low toxicity,it is widely used in biological detection field.Therefore,based on Upconversion Luminescence Nanoparticles(UCNPs),this study constructed a rapid detection technology for hormone pollutants.Objective:This study intends to explore the detection method of hormone pollutants by establishing fluorescence sensing detection technology,so as to realize the rapid detection of target objects.A chemical fluorescence sensing technology was established for the detection of bisphenol A by using the fluorescence resonance energy transfer system of potassium permanganate and up-converted nanoparticles.Abstract:A novel sensor technology for detecting 17?-estradiol,bisphenol A and progesterone in hormone pollutants was established based on the fluorescence internal filtration effect of gold nanoparticles on up-converted nanoparticles,which was used as the target specific recognition element.Design and Setting:1.Preparation of upconversion multicolor nanoparticles:Using the solvothermal method,?-Na REF₄ crystal nucleus were firstly synthesized,and then?-Na REF₄@?-Na REF₄ nanoparticles were synthesized.After the homogeneous shell layer structure encapsulation,the crystals gradually grew into the hexagonal phase,while the size of the particles also increased significantly,resulting in the synthesis of small-sized,good water-soluble,hexagonal-phase upconversion nanoparticles.By adjusting the doping ratio of rare earth elements,multicolor upconversion nanoparticles such as orange,cyan and green were formed.The prepared homogeneous core-shell structure of upconversion multicolor nanoparticles were characterized by transmission electron microscopy and fluorescence spectroscopy.2.Establishment of a chemical fluorescence sensing detection method based on UCNPs@KMn O₄:A fluorescence resonance energy transfer system based on up-conversion nanoparticles and potassium permanganate was established.The p H and reaction time of buffer solution were optimized,and A method for detecting bisphenol A was developed.The detection limit and recovery rate were evaluated.3.Preparation of adapter-upconversion nanoparticles:The upconverted multicolor nanoparticles were surface functionalized and modified,and then coupled with the target aptamer to prepare adapter-upconversion nanoparticles;gold nanoparticles were coupled with complementary chains,and the fluorescence resonance energy transfer sensing method was established by using the fluorescence internal filtration effect between upconverted nanoparticles and gold nanoparticles,and the prepared particles were characterized by transmission electron microscopy,fluorescence spectroscopy,etc.4.Establishment of aptamer-based multicolor upconversion fluorescence sensing assay:The hybridization buffer type,p H and aptamer concentration were optimized,and the aptamer-based multicolor upconversion fluorescence sensing assay was established and tested in real samples.Result:1.Establishment of a chemical fluorescence sensing detection method based on UCNPs@KMn O₄:the linear range of bisphenol A(BPA)was 228.29?68478?g/m L(y=799.85+6.46x,R²=0.999),the detection limit was 198.61?g/m L,and the recoveries were 97.72%?99.53%.2.Establishment of aptamer-based multicolor upconversion fluorescence sensing assay:the linear range of 17?-estradiol(E₂)was 100?200 ng/m L(y=11000.06 lnx-48307.31,R²=0.997),and the detection limit was 1.00 ng/m L.The linear range of bisphenol A(BPA)was 5?120 ng/m L(y=1333.60+17.68x,R²=0.996),and the detection limit was 3.55 ng/m L.The linear range of progesterone(P4)was0.0001?1000 ng/m L(y=521.68+114.76lgx,R²=0.988),the detection limit was0.0001ng/m L,the specificity was strong,and the standard experiment showed that the average recovery was good,E₂ was 99.65?110.47%,BPA was 93.90?106.22%,P4 was96.07?107.36%,the relative standard deviation was less than 8.01%.Conclusion:In this study,two kinds of fluorescence sensing detection technologies were constructed for the detection of hormone pollutants.The detection methods are accurate,efficient,simple and practical.To a certain extent,this study provides technical support for the rapid detection of hormone pollutants,which has a good theoretical value and application prospect.