Chemiluminescence-based Nanosystem For In Vivo NIR Tumor Imaging And Hydrogen Peroxide Detection

ObjectiveTo prepare and optimize a novel chemiluminescence-based integrated nanosystem for NIR tumor imaging and in vivo hydrogen peroxide detection.MethodsThe nanosystem was prepared via micelles.The components were screened by comparison of luminescence intensity and duration by the multifunctional microplate reader,and then gradually optimized to obtain the best specific dosage of each component.Next,the nanosystem was characterized for stability,absorption spectra,particle size and imaging depth.In addition,the effects of temperature and hydrogen peroxide concentration on the luminescence properties were explored.Finally,in vivo hydrogen peroxide detection imaging as well as tumor imaging performance was assessed in deep muscle and intraperitoneal tissues of mice.ResultsThe stability and luminescence intensity of the nanosystem were greatly improved with the help of DOS and surfactant Tween-80,and still responded rapidly to external hydrogen peroxide.The half-life of the nanosystem luminescence is about 8 min at37°C with 7.55 m M hydrogen peroxide,and the imaging depth is up to 10 mm.In the subcutaneous tumor model,the chemiluminescence signal was clearly distinguishable from normal tissue by rapid imaging after in situ injection of glucose oxidase and nanosystem,and the signal difference was more significant after intraperitoneal injection of exogenous glucose.ConclusionsThe nanosystem obtained by rational design and full optimization can respond to endo-exogenous hydrogen peroxide in vivo by high-efficiency NIR chemiluminescence.This nanosystem is simple to synthesize,and no complex energy transfer steps are needed to realize NIR-II imaging.It has high tissue penetration depth,extremely fast response speed as well as good biocompatibility in in vivo imaging.It has great potential for application in the diagnosis and treatment of hydrogen peroxide-related diseases.