Effects Of Lychee Rose Wine On Oxidative Damage Induced By D-galactose In Mice

Fruit wine is a kind of low alcohol beverage wine made from fresh fruit or fruit juice by whole fermentation or partial fermentation.The fruit wine retains part of the nutrition active substance,long-term drinking has certain health effect.Lychee and rose are medicinal ingredients rich in various functional ingredients,with anti-oxidation,anti-inflammatory and immune-regulating effect.In the early stage,our research group cooperated with a company and made use of modern brewing technology to produce a Lychee Rose Wine with pure aroma and pleasant taste by optimizing the brewing process.However,it remains to be further studied whether the Lychee Rose Wine retains the original nutrients and active ingredients of lychee and rose,and whether long-term drinking has antioxidant effects on the body.The aim of this study is to clarify the nutritional composition and functional components of Lychee Rose Wine,and to explore the antioxidant and health effect of Lychee Rose Wine by establishing the subacute D-galactose induced oxidative damage model in mice,so as to provide valuable theoretical basis for product development and quality control.Specific research contents and results are as follows:Firstly,the main nutrients and active components of Lychee Rose Wine were determined.The results show that the Lychee Rose Wine contains 0.23 g/100 m L protein,0.79 g/100 m L crude fat,2.56 g/100 m L total sugar and 1.97 g/100 m L reducing sugar(accounting for 76.95%of the total sugar content),which is a kind of low sugar and low fat food.The contents of plant active ingredients in Lychee Rose Wine are relatively rich,including polyphenols and flavonoids,tannins,alkaloids and anthocyanins,which are 430.00?g/m L and 254.32?g/m L,123.25?g/m L,0.66 g/100 m L and 0.42?g/m L,respectively.The contents of organic acids are rich,including malic acid 156.24 mg/L,acetic acid 138.64 mg/L,lactic acid 164.08 mg/L and citric acid 128.46 mg/L.The contents of Asp,Glu,Ala and Pro are higher in 18 kinds of amino acids.Vitamin C content is rich,up to 3.15 mg/100 m L.The main mineral elements are Ca,Mg,K and P,and the contents are 76.21 mg/L,58.35 mg/L,69.88 mg/L and 70.59 mg/L,respectively.Then,the polyphenol compounds in Lychee Rose Wine were determined.The main phenolic compounds are luteolin,protocatechin,catechin,epicatechin,gallic acid,resveratrol and ferulic acid by UPLC-QTOF-MS analysis.HPLC-PDA quantitative results show that luteolin(1.63?g/m L),catechin(2.01?g/m L),gallic acid(37.77?g/m L),resveratrol(2.35?g/m L)and ferulic acid(3.52?g/m L)are more abundant than those of the six fruit wines sold in the market.The antioxidant capacity of Lychee Rose Wine is significantly higher than that of Lychee and Blueberry wine(P<0.05),which is similar to that of Rose wine and Kiwi wine.The clearance rate of ABTS·⁺is significantly higher than that of Lychee,Blueberry and Cabernet sauvignon(P<0.05),which is similar to that of Merlot.The scavenging capacity of·OH and O₂^-·free radical is significantly higher than that of commercial Lychee wine,Rose wine and wine(P<0.05).The Fe³⁺reducing capacity and total antioxidant capacity of Lychee wine are significantly higher than those of Rose wine(P<0.05).Comprehensive evaluation show that Lychee Rose Wine has strong free radical scavenging ability and antioxidant activity in vitro.Finally,the ameliorative effects of Lychee Rose Wine on D-galactose induced oxidative damage in mice were investigated.Sixty ICR male mice were randomly divided into 6 groups:normal group,D-galactose group,Vc control group(fed 450 mg/kg Vc),low-dose,medium-dose and high-dose lychee rose wine groups(fed 10,20 and 40 m L/kg lychee rose wine,respectively).The results show that compared with D-galactose group,high dose Lychee Rose Wine could effectively reduce the levels of inflammatory factors(TNF-?,IL-1,IL-6)in plasma,ROS and MDA contents in plasma,liver,kidney,muscle and skin tissues of oxidized mice.It enhance the activities of antioxidant enzymes(GSH-Px,SOD and CAT)(P<0.05),and has better antioxidant protection on skin.Further analysis show that feeding high-dose Lychee Rose Wine could restore skin moisture content,hydroxyproline acid and collagen content to a certain extent,and skin tissue structure,dermis thickness and collagen fiber content are partially restored.RT-q PCR results show that the skin improvement effect of Lychee rose wine may be related to the regulation of collagen synthesis genes(TGF?1,Col1a1,NF-?B,AP-1,MMP-1,MMP-8).The up-regulation of antioxidant GSK-3?/Nrf2 signaling pathway and expression levels of related genes(HO-1 and NQO1)are related(P<0.05).In conclusion,Lychee Rose Wine retains rich polyphenols,flavonoids and other active ingredients as well as vitamin C,and has strong antioxidant capacity in vitro.The appropriate dose of Lychee Rose Wine could activate GSK-3?/Nrf2 signaling pathway induced by D-galactose in mice,improve the activity of antioxidant enzymes in tissues,and reduce collagen decomposition.At the same time,it can reduce the level of plasma inflammatory factors,induce TGF-?expression,improve redox homeostasis,skin moisture content,hydroxyproline and collagen content,and has a certain antioxidant effect.